Anti-PEG Antibodies

PEG (polyethylene glycol) is a nontoxic, water-soluble, biocompatible polymer that has been approved by the FDA for human intravenous, oral and dermal applications. Attachment of PEG to proteins, peptides, liposomes, drugs, and nanoparticles can improve the pharmacokinetic properties and diminish the potential for immunogenicity. Even though PEG is generally considered safe in animals and humans, the slow clearance of large PEGs is still a great concern due to the potential adverse effects resulting from PEG accumulation in tissues following chronic administration.

To allow sensitive measurement of PEGylated compounds, Creative Diagnostics have produced a set of specific anti-PEG monoclonal antibodies. These antibodies have been validated to work in various types of immunoassays and can be supplied as high quality research tools to develop sensitive and specific assays.

Cat#	Product Name	Description	Application
Anti-PEG Antibodies List
DMABT-Z60095	Rabbit Anti-PEG Mab, IgG, Biotin	Detects both linear and branched PEG.	IHC-P, ELISA, WB
DMABT-Z60369	Rabbit Anti-PEG Mab, IgM	Binds to the PEG backbone.	WB, ELISA
DMABT-Z60567	Rat Anti-PEG Mab, IgM	Displays high affinity to both short and long PEG molecules.	WB, ELISA
CABT-L3139	Biotinylated DMABT-Z60567		WB, ELISA
CABT-L3126	Mouse Anti-PEG Mab, IgG1	Detects repeating subunits present in PEG.	ELSIA, WB, IHC, FC
CABT-L3127	Mouse Anti-PEG Mab, IgG1	Binds to the PEG backbone. Detects PEGylated compounds.	ELSIA, WB, IHC
CABT-L3133	Biotinylated CABT-L3127	Binds to the PEG backbone. Detects PEGylated compounds.	ELSIA, WB, IHC
CABT-L3128	Mouse Anti-PEG Mab, IgG1	Detects backbone of PEG molecule. Detects PEGylated compounds.	ELISA, WB, FC
CABT-L3134	Biotinylated CABT-L3128		ELISA, WB, FC
CABT-L3129	Mouse Anti-mPEG Mab, IgG2b	Detects the terminal methoxy group of PEG. Suitable for detection of PEGylated liposomes and nanoparticles.	ELISA, WB, FC
CABT-L3136	Biotinylated CABT-L3129		ELISA, WB, FC
CABT-L3130	Rat Anti-PEG Mab, IgG2a	Detects PEGylated compounds. Higher affinity, Wider binding range than CABT-L3128.	ELISA
CABT-L3135	Biotinylated CABT-L3130		ELISA
CABT-L3131	Mouse Anti-PEG Mab, IgM	Binds to the PEG backbone, detects PEGylated compounds.	ELSIA, WB, IHC, FC
CABT-L3137	Biotinylated CABT-L3131	Binds to the PEG backbone, detects PEGylated compounds.	ELSIA, WB, IHC, FC
CABT-L3132	Mouse Anti-PEG Mab, IgM	Recognizes PEG backbone. Binds to PEGylated nanoparticles or long-chain PEGylated molecules.	WB, ELISA, IHC, FC
CABT-L3138	Biotinylated CABT-L3132		WB, ELISA, IHC, FC
Human Anti-PEG Standards
CABT-L3140	Chimeric Human Anti-PEG Mab, IgG1	Fusion protein of mouse CABT-L3127 VH and VL with human IgG1.
CABT-L3141	Chimeric Human Anti-PEG Mab, IgM	Fusion protein of mouse CABT-L3132 VH and VL with human IgM.
CABT-L3142	Humanized Anti-PEG Mab, IgG1	Recombinant antibody composed of humanized CABT-L3128 VH and VL with human IgG1 constant regions.
Combination Packages
CABT-L3143	Chimeric Anti-PEG Mab (Set)	This set contains 4 chimeric antibodies composed of the CABT-L3127 variable region genes fused to different human IgG constant domains (IgG1, IgG2, IgG3, and IgG4).
CABT-L3144	Chimeric Anti-PEG Mab (Set)	CABT-L3143; CABT-L3141.
IgG Package 1	Includes 100 µg of each clone	CABT-L3126; CABT-L3127; CABT-L3128; CABT-L3130; CABT-L3129.
IgM Package 1	Includes 100 µg of each clone	CABT-L3131; CABT-L3132; DMABT-Z60567.
IgG Package 2	Includes 100 µg of each clone	CABT-L3133; CABT-L3134; CABT-L3135; CABT-L3136.
IgM Package 2	Includes 100 µg of each clone	CABT-L3137; CABT-L3138; CABT-L3139.

Performance

Recognize PEG Conjugates and Free Form PEG.
Serve as Positive Controls for Drug Detection Assays.
Recommended antibody pair for sandwich ELISA (Capture-Detection):
- CABT-L3132 - CABT-L3133;
- DMABT-Z60567 - CABT-L3134

With team of antibody experts working in a state-of-the-art laboratory, Creative Diagnostics is proud to announce that a new set of PEG antibodies and standards are now commercially available. The launched products are for research purposes only, not for use in diagnostics.