PEG (polyethylene glycol) is a nontoxic, water-soluble, biocompatible polymer that has been approved by the FDA for human intravenous, oral and dermal applications. Attachment of PEG to proteins, peptides, liposomes, drugs, and nanoparticles can improve the pharmacokinetic properties and diminish the potential for immunogenicity. Even though PEG is generally considered safe in animals and humans, the slow clearance of large PEGs is still a great concern due to the potential adverse effects resulting from PEG accumulation in tissues following chronic administration.
To allow sensitive measurement of PEGylated compounds, Creative Diagnostics have produced a set of specific anti-PEG monoclonal antibodies. These antibodies have been validated to work in various types of immunoassays and can be supplied as high quality research tools to develop sensitive and specific assays.
| Anti-PEG Antibodies List | |||
| Cat# | Product Name | Description | Application |
|---|---|---|---|
| DMABT-Z60095 | Rabbit Anti-PEG Mab, IgG, Biotin | Detects both linear and branched PEG. | IHC-P, ELISA, WB |
| DMABT-Z60369 | Rabbit Anti-PEG Mab, IgM | Binds to the PEG backbone. | WB, ELISA |
| DMABT-Z60567 | Rat Anti-PEG Mab, IgM | Displays high affinity to both short and long PEG molecules. | WB, ELISA |
| CABT-L3139 | Biotinylated DMABT-Z60567 | ||
| CABT-L3126 | Mouse Anti-PEG Mab, IgG1 | Detects repeating subunits present in PEG. | ELSIA, WB, IHC, FC |
| CABT-L3127 | Mouse Anti-PEG Mab, IgG1 | Binds to the PEG backbone. Detects PEGylated compounds. | ELSIA, WB, IHC |
| CABT-L3133 | Biotinylated CABT-L3127 | ||
| CABT-L3128 | Mouse Anti-PEG Mab, IgG1 | Detects backbone of PEG molecule. Detects PEGylated compounds. | ELISA, WB, FC |
| CABT-L3134 | Biotinylated CABT-L3128 | ||
| CABT-L3129 | Mouse Anti-mPEG Mab, IgG2b | Detects the terminal methoxy group of PEG. Suitable for detection of PEGylated liposomes and nanoparticles. | ELISA, WB, FC |
| CABT-L3136 | Biotinylated CABT-L3129 | ||
| CABT-L3130 | Rat Anti-PEG Mab, IgG2a | Detects PEGylated compounds. Higher affinity, Wider binding range than CABT-L3128. | ELISA |
| CABT-L3135 | Biotinylated CABT-L3130 | ||
| CABT-L3131 | Mouse Anti-PEG Mab, IgM | Binds to the PEG backbone, detects PEGylated compounds. | ELSIA, WB, IHC, FC |
| CABT-L3137 | Biotinylated CABT-L3131 | ||
| CABT-L3132 | Mouse Anti-PEG Mab, IgM | Recognizes PEG backbone. Binds to PEGylated nanoparticles or long-chain PEGylated molecules. | WB, ELISA, IHC, FC |
| CABT-L3138 | Biotinylated CABT-L3132 | ||
| Human Anti-PEG Standards | |||
| CABT-L3140 | Chimeric Human Anti-PEG Mab, IgG1 | Fusion protein of mouse CABT-L3127 VH and VL with human IgG1. | |
| CABT-L3141 | Chimeric Human Anti-PEG Mab, IgM | Fusion protein of mouse CABT-L3132 VH and VL with human IgM. | |
| CABT-L3142 | Humanized Anti-PEG Mab, IgG1 | Recombinant antibody composed of humanized CABT-L3128 VH and VL with human IgG1 constant regions. | |
| Combination Packages | |||
| CABT-L3143 | Chimeric Anti-PEG Mab (Set) | This set contains 4 chimeric antibodies composed of the CABT-L3127 variable region genes fused to different human IgG constant domains (IgG1, IgG2, IgG3, and IgG4). | |
| CABT-L3144 | Chimeric Anti-PEG Mab (Set) | CABT-L3143; CABT-L3141. | |
| IgG Package 1 | Includes 100 µg of each clone | CABT-L3126; CABT-L3127; CABT-L3128; CABT-L3130; CABT-L3129. | |
| IgM Package 1 | Includes 100 µg of each clone | CABT-L3131; CABT-L3132; DMABT-Z60567. | |
| IgG Package 2 | Includes 100 µg of each clone | CABT-L3133; CABT-L3134; CABT-L3135; CABT-L3136. | |
| IgM Package 2 | Includes 100 µg of each clone | CABT-L3137; CABT-L3138; CABT-L3139. | |
Performance
With team of antibody experts working in a state-of-the-art laboratory, Creative Diagnostics is proud to announce that a new set of PEG antibodies and standards are now commercially available. The launched products are for research purposes only, not for use in diagnostics.
