Application: We recommend the following antibodies for sandwich immunoassay (Capture - Detection): DMAB-CPT465 - DMAB-CPT466
Summary
Specifications
Antibody Isotype
IgG
Clone
341212B4
Species Reactivity
Mouse
Immunogen
Hif1a fusion protein
Conjugate
Unconjugated
Applications
Application Notes
We recommend the following antibodies for sandwich immunoassay (Capture - Detection): DMAB-CPT467 - DMAB-CPT468 Sandwich ELISA: 0.156-10 ng/mL
General Notes
The Magic™ Rabbit Anti-Mouse HIF1A Monoclonal Antibody (clone 341212B4, product code DMAB-CPT467) is a rabbit monoclonal antibody specifically directed against mouse HIF1A (Hypoxia Inducible Factor 1 Alpha). HIF1A is a key transcription factor that mediates cellular responses to hypoxic conditions by regulating the expression of genes involved in angiogenesis, metabolism, erythropoiesis, and survival pathways. This antibody is designed for the specific detection of mouse HIF1A and is supplied in an unconjugated format suitable for immunoassay development. This product is validated for use in sandwich ELISA (capture format) as part of a matched antibody pair system. The recommended pairing is DMAB-CPT467 (capture) with DMAB-CPT468 (detection), enabling sensitive sandwich ELISA detection with a working range of 0.156–10 ng/mL. It is suitable for quantitative detection of HIF1A in mouse biological samples and optimized for robust ELISA-based immunodetection workflows requiring high specificity and sensitivity. DMAB-CPT467 is a rabbit IgG monoclonal antibody purified using Protein A affinity chromatography and supplied as a liquid formulation in PBS only. Each vial contains 100 μg of purified antibody. It is stored at −80°C for long-term preservation.
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