Sample
serum, plasma, cell culture supernatants, tissue homogenate
Detection Method
Sandwich ELISA
Intended Use
For quantitative detection of Mouse Bnip3 (BCL2/adenovirus E1B 19 kDa protein-interacting protein 3) in serum, plasma, tissue homogenates and other biological fluids.
Contents of Kit
1. 96-well strip plate (Dismountable), 1 plate
2. Lyophilized Standard, 2 vials
3. Sample/Standard dilution buffer, 20 mL
4. Biotin-detection antibody (Concentrated), 120 uL
5. Antibody dilution buffer, 10 mL
6. HRP-Streptavidin Conjugate(SABC), 120 uL
7. SABC dilution buffer, 10 mL
8. TMB substrate, 10 mL
9. Stop solution, 10 mL
10. Wash buffer (25X), 30 mL
11. Plate Sealer, 5 pieces
12. Product Manual, 1 copy
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Precision
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Detection Range
78-5000 pg/mL
Citations
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Li, LH; Qu, Y; et al. Influence of hypoxia-inducible factor 1-alpha on neuronal apoptosis in a rat model of hypoxia- or hypoxia-ischemia-induced brain injury. NEURAL REGENERATION RESEARCH 4:1019-1023(2009).
Kalas, W; Swiderek, E; et al. Thrombospondin-1 Receptor Mediates Autophagy of RAS-expressing Cancer Cells and Triggers Tumour Growth Inhibition. ANTICANCER RESEARCH 33:1429-1438(2013).