Mouse B. pertussis (FHA) IgG ELISA kit (DEIASL129)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
Serum, plasma
Species Reactivity
Mouse
Intended Use
The Mouse Anti-B. Pertussis FHA IgG ELISA Kit detects and quantifies B. pertussis FHA-specific IgG in mouse serum or plasma of vaccinated or immunized animals. This immunoassay is suitable for:
o Determining immune status relative to non-immune controls;
o Assessing efficacy of vaccines, including dosage, adjuvantcy, route of immunization and timing;
o Qualifying and/or standardizing vaccine batches and protocols.
The kit contains no active toxin or inactivated bacteria. The antigen used is highly purified pertussis toxoid (non-toxic). For in vitro research use only (RUO), not for therapeutic or diagnostic use.
Storage
The microtiter well plate and all other reagents, if unopened, are stable at 2-8°C until the expiration date printed on the box label.
Sensitivity
The FHA coating level, HRP conjugate concentration and Low NSB Sample Diluent are optimized to differentiate anti-FHA IgG from background (non-antibody) signal with mouse serum samples diluted 1:100.
General Description
Pertussis, also known as Whooping Cough, is a highly contagious disease caused by Bordetella pertussis bacteria. Vaccines for pertussis, available in combination with vaccines for tetanus, diphtheria, H. influenza b, hepatitis & polio, use acellular components, primarily the inactivated pertussis toxin. The toxin, a protein exotoxin, produced only by B. pertussis, is central to pertussis pathogenesis; vaccination with the toxoid elicits high levels of protection from the disease. Also included are two other highly immunogenic pertussis proteins: pertactin, an outer membrane protein that promotes adhesion to host cells, and filamentous hemagglutinin (FHA).

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References


Lee, LH; Blake, MS; et al. Effect of Increased CRM197 Carrier Protein Dose on Meningococcal C Bactericidal Antibody Response. CLINICAL AND VACCINE IMMUNOLOGY 19:551-556(2012).
Khatami, A; Snape, MD; et al. Persistence of Immunity Following a Booster Dose of Haemophilus Influenzae Type B-Meningococcal Serogroup C Glycoconjugate Vaccine Follow-up of a Randomized Controlled Trial. PEDIATRIC INFECTIOUS DISEASE JOURNAL 30:197-202(2011).

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