Contents of Kit
1. Polystyrene microwell ELISA plates coated with a purified antigen(12-1 x 8 wells), with holder in foil package containing desiccants
2. Negative Control, 1 vial of buffer 1.2 mL
3. Positive Control, 1 vial of buffer 1.2 mL
4. Calibrator A, 1 vial of buffer containing preservative, prediluted, 1.2 mL
5. Calibrator B, 1 vial of buffer containing preservative, prediluted, 1.2 mL
6. Calibrator C, 1 vial of buffer containing preservative, prediluted, 1.2 mL
7. Calibrator D, 1 vial of buffer containing preservative, prediluted, 1.2 mL
8. Sample Diluent, 1 vial – colored straw containing PBS-buffered saline, protein stabilizers and preservative, 50 mL.
9. Antibody Enzyme Conjugate, colored blue containing buffer, protein stabilizers and preservative, 12 mL
10. Wash Buffer (20 ×), 50 mL
11. TMB Chromogen, containing stabilizers, 10 mL
12. Stop Solution, Colorless, 10 mL
General Description
Autoimmune hepatitis is characterized by chronic inflammation of the liver, leading to progressive liver damage and cirrhosis if left untreated. This condition is driven by an autoimmune attack on liver cells. Key autoantibodies for diagnosis include:
Antinuclear Antibodies (ANA)
Anti-Smooth Muscle Antibodies (ASMA)
Anti-Liver Kidney Microsomal Type 1 Antibodies (LKM-1)
Anti-Soluble Liver Antigen Antibodies (SLA)
Anti-smooth muscle antibodies are present in up to 70% patients with autoimmune chronic active hepatitis and in 25-40% of patients with primary biliary cirrhosis (often together with anti-mitochondrial antibodies). Low titres of anti-smooth muscle antibodies are also associated with infectious diseases giving hypogammaglobulinaemia. Lower levels (usually) are also found in connective tissue diseases and chronic infections. The antigen recognised by anti-smooth muscle antibodies in sera of patients with Type 1 chronic active hepatitis is often reported to be actin.
COA
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