Contents of Kit
1. Polystyrene microwell ELISA plates coated with a purified antigen(12-1 x 8 wells), with holder in foil package containing desiccants
2. Negative Control, 1 vial of buffer 1.2 mL
3. Positive Control, 1 vial of buffer 1.2 mL
4. Calibrator A, 1 vial of buffer containing preservative, prediluted, 1.2 mL
5. Calibrator B, 1 vial of buffer containing preservative, prediluted, 1.2 mL
6. Calibrator C, 1 vial of buffer containing preservative, prediluted, 1.2 mL
7. Calibrator D, 1 vial of buffer containing preservative, prediluted, 1.2 mL
8. Sample Diluent, 1 vial – colored straw containing PBS-buffered saline, protein stabilizers and preservative, 50 mL.
9. Antibody Enzyme Conjugate, colored blue containing buffer, protein stabilizers and preservative, 12 mL
10. Wash Buffer (20 ×), 50 mL
11. TMB Chromogen, containing stabilizers, 10 mL
12. Stop Solution, Colorless, 10 mL
General Description
SS-A is a RNA protein particle (ribonucleoprotein, RNP) ubiquitously distributed in all tissues. It is composed of two proteins (60kDa and 52kDa) associated with at least four small uridin-rich cytoplasmic RNAs (hyRNA, human cytoplasmic RNA); its function is yet unknown. Autoantibodies against SS-A (formerly named Ro after prototype subject Robert) as well as SSB (formerly named La after prototype subject Lane) are typical markers for studying Sjögren`s syndrome (SS) and systemic lupus erythematosus (SLE).