Contents of Kit
1. Polystyrene microwell ELISA plates coated with a purified antigen(12-1 x 8 wells), with holder in foil package containing desiccants
2. Negative Control, 1 vial of buffer 1.2 mL
3. Positive Control, 1 vial of buffer 1.2 mL
4. Calibrator A, 1 vial of buffer containing preservative, prediluted, 1.2 mL
5. Calibrator B, 1 vial of buffer containing preservative, prediluted, 1.2 mL
6. Calibrator C, 1 vial of buffer containing preservative, prediluted, 1.2 mL
7. Calibrator D, 1 vial of buffer containing preservative, prediluted, 1.2 mL
8. Sample Diluent, 1 vial – colored straw containing PBS-buffered saline, protein stabilizers and preservative, 50 mL.
9. Antibody Enzyme Conjugate, colored blue containing buffer, protein stabilizers and preservative, 12 mL
10. Wash Buffer (20 ×), 50 mL
11. TMB Chromogen, containing stabilizers, 10 mL
12. Stop Solution, Colorless, 10 mL
General Description
The anti-nuclear antigens (ANA) can be divided into three groups:
1.true anti nuclear antigens (ANA): dsDNA, ssDNA, histones, nucleolic RNA and DNP
2.extractable nuclears antigens: Sm (Smith), n-RNP, Scl 70 and PM-1
3.cytoplasmatic antigens: SS-A (Ro)*, SS-B (La)* and Jo-1 SS-A (Ro) and SS-B (La) are co-localized in cytoplasm and nucleus
Anti-Sm/RNP antibodies are a subset of antinuclear antibodies (ANA) that target specific nuclear antigens, including the Smith antigen (Sm) and ribonucleoprotein (RNP) complexes. These antibodies are particularly significant in the diagnosis and management of systemic lupus erythematosus (SLE) and mixed connective tissue disease (MCTD).
Anti-Sm antibodies are highly specific for SLE, although they are not as frequently detected as other ANAs. Their presence strongly supports the diagnosis of SLE.
Anti-RNP antibodies are a hallmark of MCTD, a condition characterized by features of SLE, systemic sclerosis, and polymyositis. These antibodies target the U1-RNP complex, which includes several proteins and RNA molecules.
COA
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