Mouse Anti-2019 nCoV(S)IgA ELISA Kit

Sample

Serum, plasma, cell culture supernatant and other biological samples

Species Reactivity

Mouse

Detection Method

iELISA

Intended Use

In vitro quantitative determination of Anti-2019 nCoV (S) IgA concentrations in serum, plasma, cell culture supernatant and other biological samples.

Contents of Kit

The sealed kit can be stored at 2-8 °C. The storage condition for opened kit is specified in the table below:
1. ELISA Microplate(Dismountable), 8×12. Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C. Stored for 12 month at -20°C
2. Lyophilized Standard, 2vial. Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C. Stored for 12 month at -20°C
3. HRP-labeled Antibody (Concentrated, 100×), 60μl, 2-8°C (Avoid Direct Light)
4. TMB Substrate, 5ml, 2-8°C (Avoid Direct Light)
5. Sample Dilution Buffer, 20ml, 2-8°C
6. Antibody Dilution Buffer, 5ml, 2-8°C
7. Stop Solution, 5ml, 2-8°C
8. Wash Buffer(25×), 30ml, 2-8°C
9. Plate Sealer, 5 pieces
10. Product Description, 1 copy
Note: The liquid reagent bottle contains slightly more reagent than indicated on the label. Please use pipette accurately measure and do proportional dilution.

Storage

2-8°C (for sealed box), please do not freeze! See kit label for expiry date

Performance Characteristics

Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

Precision

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

Detection Range

0.156-10ng/ml

Sensitivity

0.094ng/ml

General Description

2019-nCoV (2019-NCoV) is a new coronavirus, which belongs to β-CoV of the reticulariviridae coroniviridae with SARS-CoV. It is an unsegmented single-stranded positive RNA virus with a genome length of about 30,000 nucleotides per group. In terms of gene sequence homology, the 2019-nCoV genome has 80% similarity with SARS, and the 2019-nCoV gene sequence has 40% similarity with MERS-CoV gene sequence. S protein is the most important membrane protein on the surface of coronavirus. Studies have shown that the S protein is closely related to the process of coronavirus invasion into cells. N protein is another important structural protein in coronaviruses. In the coronavirus particle, the N protein is at the core of the virus particle and exists in the form of Genomic RNA.

Standard Curve

This product has been tested by Quality Control Department and meets performance specifications mentioned in the manual. (The humidity in the laboratory is 20%-60%, and the temperature is 18°C -25°C. TMB was balanced to 37°C before color development, and incubated at 37°C for 15 minutes in the dark after adding the enzyme label plate holes.)
The following assay data are provided for reference, since experimental environment and operation are different. The establishment of standard curve depends on your own assay.