Intended Use
CD's Varicella zoster IgM Antibody ELISA Test Kit has been designed for the detection of IgM class antibodies against Varicella zoster in monkey (rhesus, cynomolgous, and baboon) serum or plasma (Citrate, heparin). This kit is for in-vitro research use only (RUO) and not for diagnosis cure or prevention of the disease.
Storage
The microtiter well plate and all other reagents are stable at 2-8°C until the expiration date printed on the label. The whole kit stability is usually 12 months from the date of shipping under appropriate storage conditions. The unused portions of the standards should be stored at 2-8°C or stored frozen in small aliquots and should be stable for 3 months.
General Description
Varicella-zoster virus (VZV) is known by many names, including chickenpox virus, varicella virus, zoster virus, and human herpes virus type 3 (HHV-3). VZV is closely related to the herpes simplex viruses (HSV), sharing much genome homology. It is one of eight herpes viruses known to infect humans and other vertebrates. It commonly causes chickenpox in children and adults and herpes zoster (shingles) in adults and rarely in children. As with the other herpes viruses, VZV causes both acute illness and lifelong latency. Before vaccination became widespread, acute primary infection (varicella or"chickenpox") was common during childhood--especially in temperate climates. Varicella usually is a benign and self-limiting illness but can be more severe in adults and in individuals with cellular immunodeficiency. These individuals are at much higher risk of pneumonia and disseminated disease with visceral involvement. Zoster typically presents as a painful, localized cutaneous eruption occurring along 1 or more contiguous dermatomes. Humans are the only known natural hosts of VZV. Transmission of VZV occurs through direct contact with infectious lesions or by inoculation of aerosolized infected droplets onto a susceptible mucosal surface.
The known envelope glycoproteins (gB, gC, gE, gH, gI, gK, gL) correspond with those in HSV; however, there is no equivalent of HSV gD. The most popular test detects VZV specific IgM antibody in blood; this appears only during chickenpox or herpes zoster and not while the virus is dormant.
Varicella Virus Infection in monkey
Human VZV is specific for human host. Simian varicella virus (SVV), the etiologic agent of naturally occurring varicella in primates, is genetically and antigenically closely related to human varicella zoster virus (VZV). Experimental inoculation of mice, rats and nonhuman primates (NHP) with VZV results in seroconversion but not varicella. There is a scarcity of the human, mouse (animal) and simian antibody ELISA kits so we do not know the extent of antibody cross reactivity between human and simian VZV antigens.
COA
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