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Leptospira hardjo is a pathogenic serovar of the Leptospira genus, a group of spiral-shaped, Gram-negative bacteria responsible for leptospirosis—a zoonotic disease that affects both animals and humans. This serovar is particularly associated with cattle, causing reproductive disorders such as abortion, stillbirths, and reduced milk production, which result in significant economic losses in the livestock industry. Additionally, Leptospira hardjo can be transmitted to humans via contact with contaminated urine or tissues from infected animals, leading to flu-like symptoms, jaundice, and in severe cases, organ failure. The Leptospira Hardjo Antibody ELISA Kit is a specialized diagnostic tool designed for the qualitative and semi-quantitative detection of IgG antibodies specific to Leptospira hardjo in serum and plasma samples from cattle and other susceptible species, as well as humans. This kit plays a pivotal role in identifying exposure to Leptospira hardjo, monitoring herd immunity, and supporting epidemiological surveillance. By detecting specific antibodies, it enables timely intervention to control outbreaks, reduce transmission risks, and protect both animal and public health.
Figure 1. Leptospira Hardjo.
Leptospira Hardjo infection in cattle is often subclinical, meaning the animals show few, if any, outward signs of sickness. This makes it a silent spreader, as infected cows can shed the bacteria in their urine for months or even years, contaminating the environment and infecting other animals. The most significant economic losses associated with Leptospira Hardjo are due to reproductive failure. This includes:
Abortion: Often occurring in the last trimester of pregnancy.
Infertility: Difficulty in conceiving or carrying a pregnancy to term.
Stillbirths and weak calves: Calves born alive may be weak and fail to thrive.
Mastitis: A unique "flabby bag" mastitis where the udder becomes soft and swollen, producing thick, yellow, colostrum-like milk, even in non-pregnant cows.
Reduced milk production: A chronic decrease in milk yield over time.
Because these signs can be attributed to a variety of other health issues, diagnosing Leptospira Hardjo based on clinical symptoms alone is nearly impossible. This underscores the need for a reliable and specific diagnostic tool.
Sample Type
Performance Metrics
Applications
Performance Characteristics
Principle of the Assay
Sample Requirements
Why Choose This ELISA Kit?
| Parameter | Details |
| Product name | Leptospira Hardjo Antibody ELISA Kit |
| Intended Use | The Leptospira hardjo (LH) IgG/IgM ELISA kit is an enzyme-linked immunosorbent assay (ELISA) for the qualitative detection of IgG and IgM antibodies to Leptospira hardjo in bovine serum. |
| Storage | The kit will remain stable until the expiration date, provided it is stored between 2-8°C. Unused strips and wells should be sealed in the foil pouch and stored as previously described. Do not freeze. |
| General Description | Leptospira serovar hardjo is endemic in cattle population, which causes economic losses in the dairy industry as a consequence of agalactia, abortion, stillbirth, birth of weak calves and reduced fertility. The hardjo infection is also an occupational zoonosis of those who work with cattle. Control schemes are being looked at in a number of countries, however, current tests are unsafe, in-sensitive, non-specific and complicated. Creative-Diagnostics has developed a highly specific and sensitive ELISA test for the detection of IgG and IgM antibodies to both leptospira borgpetersenii serovar hardjo bovis (HB) and Lepto spira interrogans serovar hardjo prajitno (HP) in sera. The hardjo IgG/IgM test kit provides a means for the qualitative detection of both IgG and IgM antibodies directed to LH in bovine serum. The test may aid in the determination of the animal's serological status, or may aid in the diagnosis of disease associated with serovar hardjo. IgM appears 2 weeks earlier than IgG, however, IgG will remain in the infected individual's system for a longer period after infection. Therefore, combining the detection of IgG and IgM antibodies will allow early detection and also detect antibodies over the whole infection period. This will allow time for effective actions against infection by Leptospira serovar hardjo to be taken. |
The Leptospira Hardjo Antibody ELISA Kit is versatile, with applications in veterinary medicine, public health, and research:
Veterinary Diagnostics: Screening cattle herds for Leptospira hardjo exposure to identify infected animals, guide vaccination strategies, and reduce reproductive losses. It is also used to monitor antibody responses post-vaccination, ensuring vaccine efficacy.
Public Health Surveillance: Detecting human exposure to Leptospira hardjo in high-risk groups (e.g., farmers, veterinarians, abattoir workers) to facilitate early intervention and prevent severe disease.
Epidemiological Studies: Assessing seroprevalence of Leptospira hardjo in animal populations to map transmission hotspots and inform control measures.
Research: Investigating the dynamics of Leptospira hardjo infection, including host immune responses and the impact of environmental factors on transmission.
Cost-Effectiveness
While the initial cost of a single ELISA kit may seem higher than a few individual tests, its high-throughput capability makes it highly cost-effective on a per-sample basis for large-scale screenings.
Safety and Ease of Use
The ELISA kit uses an inactivated, non-infectious Leptospira Hardjo antigen. This eliminates the biohazard risk associated with handling live bacterial cultures, which is a requirement for the MAT.
Objectivity
The results are read objectively by a microplate reader, which measures the optical density (OD) of the color change in each well. This numerical result eliminates the subjective interpretation inherent in older methods that rely on visual observation, such as the MAT.
The Leptospira Hardjo Antibody ELISA Kit is an essential tool for the detection of Leptospira hardjo-specific IgG antibodies, offering high sensitivity, specificity, and ease of use. By enabling rapid and reliable screening, it supports effective disease management in livestock, reduces economic losses, and protects public health by identifying human exposure risks. Whether in veterinary clinics, diagnostic laboratories, or research institutions, this kit is instrumental in combating the impact of leptospirosis caused by Leptospira hardjo.
Some kits are validated for use with sheep, goats, and pigs, but species-specific secondary antibodies are required. Check the product manual for compatibility.
Results are expressed as a sample-to-positive (S/P) ratio or titer, calculated using the absorbance values of standards. Values above the cut-off (defined in the manual) indicate positive antibody presence.
Borderline samples should be retested, and confirmation with MAT is recommended to resolve ambiguity.
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