Intended Use
This ELISA was developed with serum from rabbits immunized with Lanreotide coupled to a carrier protein (Synthetic peptide H-D-2-Nal-Cys-Tyr-D-Trp-Lys-Val-Cys-Thr-NH2 (Disulfide bond) coupled to carrier protein). This ELISA has been validated with the included reagents. It is intended to be used with appropriately extracted samples. For research use only.
Contents of Kit
1. ELISA buffer concentrate (50ml 20× concentrate)
2. 96-well immunoplate with plate sealer
3. Antiserum (lyophilized powder)
4. Standard (1mg lyophilized powder)
5. Biotinylated tracer (lyophilized powder)
6. Streptavidin-HRP (100μl 200× concentrate)
7. TMB substrate stock solution (1.5ml)
8. TMB substrate buffer (15ml citrate buffer)
9. Stop solution 2 N HCl (15 ml)
Storage
After you receive the kit, store the lyophilized components and standard diluent at -20°C for up to one year from the kit's assembly date. The remaining components should be stored in the refrigerator (4 - 8°C) also up to 1 year. Long term storage, improper storage conditions and large temperature fluctuation cycles may cause precipitates in the TMB solution and in the ELISA buffer concentrate. These precipitates should not affect the assay noticeably. Nevertheless, if you observe such precipitates, we recommend to avoid them by allowing them to sink to the bottom.
Detection Range
0-10ng/ml
General Description
Lanreotide is a synthetic peptide analogue of somatostatin, a naturally occurring inhibitory hormone which blocks the release of several other hormones, including growth hormone, thyroid-stimulating hormone (TSH), insulin and glucagon. It binds to the same receptors as somatostatin, although with higher affinity to peripheral receptors, and has similar activity. However, while somatostatin is quickly broken down in the body (within minutes), lanreotide has a much longer half-life, and produces far more prolonged effects.
Standard Curve
Typical titration curve of Lanreotide in a competitive ELISA with this antibody:
Citations
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COA
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