PCNA

Proliferating cell nuclear antigen (PCNA) is an auxiliary protein of DNA polymerase delta, forms homotrimers around double stranded DNA. It is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA is a highly conserved protein; it even occurs in some viruses. As a histone-like protein it interacts with many other nuclear proteins and is therefore ubiquitous in caryoplasm. The functional form of PCNA is a ring-shaped trimer, which encircles dsDNA. This explains why PCNA is associated with DNA in absence of a DNA binding motif. PCNA expression rate correlates with the rate of DNA replication and cell growth. The rate is significantly elevated during the S and G2 phases of the cell cycle, but is very low in quiescent cells, makes this protein a good marker for cell proliferation assay. Since then, the quantitative measurement of PCNA in human cell and tissue extracts has been extensively used in cancer diagnosis and prognosis.

More than 100 different autoantibodies have been associated with SLE and several reports highlight the diagnostic value of SLE specific antibodies, which were detected even before the onset of diagnostic symptoms. Autoantibodies against PCNA were first reported by Miyachi etal.[1] and are detected in approximately 5-10% of SLE patients. PCNA autoantibodies have been reported to be present in those patients suffering from arthritis and hypocomplementemia, and to drop below detection limits following drug treatment. If antibodies to PCNA occur in SLE, this antigen is of high diagnostic value as we can expect very small inter-individual variations.[2]

Creative Diagnostics now can provide both recombinant full length PCNA produced in the baculovirus/insect cell expression system and native PCNA. Welcome to contact us for more information.

References

  1. Latisha D. Heinlen, Lauren L. Ritterhouse, Micah T. McClain,. Ribosomal P autoantibodies are present before SLE onset and are directed against non-C-terminal peptides[J]. Journal of Molecular Medicine, 88(7):719-727.
  2. R Asero, L Origgi, S Crespi.  Autoantibody to proliferating cell nuclear antigen (PCNA) in SLE: a clinical and serological study[J]. Clinical & Experimental Rheumatology, 1987, 5(3):241-246.
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PCNA

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  • CABT-50334MP
  • Anti-PCNA monoclonal antibody, clone PC10
  • Antibody
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  • DescriptionMouse Anti-Human PCNA Monoclonal antibody for IHC-Fr, FC, IP, IHC-P, WB
    SpecificityPCNA
    HostMouse
    BufferPBS, pH 7.4, 0.1 % sodium azide
    PurityAffinity Purified.
    StorageAliquot and store at -20 °C. Avoid repeated freeze/thaw cycles
  • CABT-L2423
  • Anti-Human PCNA chimeric monoclonal antibody
  • Antibody
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  • DescriptionMouse Anti-Human PCNA chimeric Monoclonal antibody for IEP, WB
    CABT-L2423, Anti-Human PCNA chimeric IgG consists of the human constant region of the heavy chain, mouse variable region of the heavy chain and mouse light chain. The human constant region of the heavy chain can be directly recognized by the Anti-Human conjugate, which is used in numerous in vitro diagnostic assays.
    SpecificityThis antibody react with human PCNA
    HostMouse
    BufferIMDM, 10% FCS, 1% protein-free stabilizer
    Purity﹥95% Purity.
    StorageAliquot and store at -20 °C. Avoid repeated freeze/thaw cycles
  • DAG615
  • Recombinant Human Proliferating Cell Nuclear Antigen [His]
  • Antigen
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  • DescriptionRecombinant human proliferating cell nuclear antigen with a hexa-histidine tag from insect cells.
    HostInsect cells
    BufferNeutral to slightly alkaline pH, 20% glycerol
    Purity> 90% as determined by reducing SDS-PAGE
    StorageStored at <-70°C. Avoid multiple freeze/thaw cycles
  • DMAB-DCC186
  • Anti-PCNA monoclonal antibody
  • Antibody
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  • DescriptionRabbit Anti-Human PCNA Monoclonal antibody for WB, ICC/IF, IHC, IP, FC
    SpecificityPCNA
    HostRabbit
    Buffer1×TBS (pH7.4), 1% BSA, 40% Glycerol.
    PurityProtein A Purified.
    StorageStore at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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