Angiopoietin-2 (ANG-2)

Angiopoietin belongs to the VEGF protein family involved in postnatal and embryonic angiogenesis. Angiopoietin proteins regulate the growth, chemotactic activity and functional behavior of vascular endothelial cells, and participate in angiogenesis. They are a family of growth factors, the best-studied being angiopoietin 1 (Ang-1) and angiopoietin 2 (Ang-2). Ang-1 and Ang-2 are important factors regulating endothelial vascular permeability. During normal development, they are considered essential for vascular differentiation through angiogenesis, the growth and remodeling of existing blood vessels; they also promote various functions characteristic of the mature blood vasculature, such as endothelial cell survival.

Angiopoietin in diagnostics

  • Angiopoietin-1 and angiopoietin-2 as clinically informative prognostic biomarkers of morbidity and mortality in severe sepsis. The abnormal expression of angiopoietin proteins also related to the occurrence of various diseases, such as rheumatoid arthritis (RA), tumors, malaria, pulmonary hypertension, etc.
  • Elevation of Angiopoietin-2 commonly occurs in patients with angiosarcoma.
  • Serum Ang-2 is considered a predictive and prognostic biomarker for immune checkpoint therapy and may contribute to treatment resistance by increasing proangiogenic and immunosuppressive activities in the tumor microenvironment.
  • Serum levels of angiopoietin-2 expression are associated with the growth of multiple myeloma, angiogenesis, and overall survival in oral squamous cell carcinoma. Circulating angiopoietin-2 is a marker for early cardiovascular disease in children on chronic dialysis.

Assay development

Ang levels in serum can be measured by sandwich ELISA using anti-Ang-1/2 antibodies (both monoclonal and polyclonal antibodies). For the development of Ang-1/2 immunoassays, Creative Diagnostics provides several related reagents.

References

  1. Ishikawa A, Okada J, Nishi K, et al. Efficacy of serum angiopoietin-1 measurement in the diagnosis of early rheumatoid arthritis[J]. Clinical & Experimental Rheumatology, 2011, 29(4):604.
  2. Tanja H, Pipsa S, D’Amico Gabriela, et al. Effects of Angiopoietin-2-Blocking Antibody on Endothelial Cell-Cell Junctions and Lung Metastasis[J]. Journal of the National Cancer Institute(6):6.
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Angiopoietin-2 (ANG-2)

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  • DCABY-3932
  • Anti-Angiopoietin-2 monoclonal antibody
  • Antibody
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  • Description Mouse Anti-Human Angiopoietin-2 Monoclonal antibody for ELISA(Cap)
    This antibody can be used as the capture when paired with CD Cat# DCABY-4263 as solid phase detection antibody.
    Specificity Detects human Angiopoietin-2 in ELISAs. In ELISAs, this antibody shows no cross-reactivity or interference with recombinant human (rh) Ang-1, rhAng-4, rhAng-X, rmAng-3, rmANGPTL-3, rhTie-1, rhTie-2, and rmTie-2.
    Host Mouse
    Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
    Purity Protein A/G Purified.
    Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
    Pair recommendations Pair with DCABY-4263
  • DCABY-4263
  • Anti-Angiopoietin-2 monoclonal antibody [Biotin]
  • Antibody
  • INQUIRY
  • Description Mouse Anti-Human Angiopoietin-2 Monoclonal antibody [Biotin] for ELISA(Det)
    This antibody can be used as the detection when paired with CD Cat# DCABY-3932 as solid phase capture antibody.
    Specificity Detects human Angiopoietin-2 inELISAs. In ELISAs, this antibody shows no cross-reactivity with recombinant human (rh) Ang-1, rhAng-4, rhAng-X, rmAng-3, rmANGPTL3, rhTie-1, rhTie-2, and rmTie-2.
    Host Mouse
    Buffer Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
    Purity Protein A/G Purified.
    Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
    Pair recommendations Pair with DCABY-3932
  • DrMAB-170
  • Recombinant Anti-Angiopoietin-2 Antibody
  • Antibody
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  • Description Recombinant Human Anti-Human Angiopoietin-2 Monoclonal antibody for ELISA, FuncS [No preservation]
    Developed by antibody phage display technology using a human naive antibody gene library.
    This clone recognizes human angiopoietin-2. Does not recognize mouse angiopoietin-2 and human angiopoietin-1. This clone inhibits the binding of human angiopoietin-2 to human Tie-2.
    Specificity Recognizes Human angiopoietin-2. Does not recognize Mouse angiopoietin-2 and Human angiopoietin-1.
    Host Human
    Buffer Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
    Purity > 95% Pure (SDS-PAGE).
    Storage Short Term (≤ 2 weeks): 2-8°C. Long Term: -20°C. Avoid repeated freezing and thawing.
  • DrMAB-171
  • Recombinant Anti-Angiopoietin-2 Antibody
  • Antibody
  • INQUIRY
  • Description Recombinant Human Anti-Human Angiopoietin-2 Monoclonal antibody for ELISA, FuncS
    Developed by antibody phage display technology using a human naive antibody gene library.
    This clone recognizes human angiopoietin-2. Does not recognize mouse angiopoietin-2 and human angiopoietin-1. This clone inhibits the binding of human angiopoietin-2 to human Tie-2.
    Specificity Recognizes Human angiopoietin-2. Does not recognize Mouse angiopoietin-2 and Human angiopoietin-1.
    Host Human
    Buffer Liquid. In PBS containing 10% glycerol
    Purity > 95% Pure (SDS-PAGE).
    Storage Short Term (≤ 2 weeks): 2-8°C. Long Term: -20°C. Avoid repeated freezing and thawing.
  • DrMAB-172
  • Recombinant Anti-Angiopoietin-2 Antibody
  • Antibody
  • INQUIRY
  • Description Recombinant Mouse Anti-Human Angiopoietin-2 antibody for ELISA, ICC, Blocking
    This antibody is composed of human variable regions (λ-chain) of immunoglobulin fused to the mouse lgG2b Fc domain. This clone recognizes human and mouse angiopoietin-2. Does not detect human angiopoietin-1.
    Specificity Recognizes Human and Mouse angiopoietin-2. Does not detect Human angiopoietin-1.
    Host Mouse
    Buffer Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
    Purity > 95% Pure (SDS-PAGE).
    Storage Short Term (≤ 2 weeks): 2-8°C. Long Term: -20°C. Avoid repeated freezing and thawing.
  • DrMAB-173
  • Recombinant Anti-Angiopoietin-2 Antibody
  • Antibody
  • INQUIRY
  • Description Recombinant Mouse Anti-Human Angiopoietin-2 antibody for ELISA, ICC, Blocking [No preservation]
    This antibody is composed of human variable regions (λ-chain) of immunoglobulin fused to the mouse lgG2b Fc domain. This clone recognizes human and mouse angiopoietin-2. Does not detect human angiopoietin-1.
    Specificity Recognizes Human and Mouse angiopoietin-2. Does not detect Human angiopoietin-1.
    Host Mouse
    Buffer Liquid. In PBS containing 10% glycerol.
    Purity > 95% Pure (SDS-PAGE).
    Storage Short Term (≤ 2 weeks): 2-8°C. Long Term: -20°C. Avoid repeated freezing and thawing.
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