Ipilimumab ELISA Kit

Sample

Serum, Plasma (EDTA, Heparin)

Species Reactivity

Human

Detection Method

sELISA

Intended Use

The Ipilimumab ELISA has been especially developed for the quantitative analysis of free ipilimumab in serum and plasma samples.

Storage

The kit is shipped at ambient temperature (10-30°C) and should be stored at 2-8°C for long term storage. Keep away from heat or direct sunlight. The strips of microtiter plate are stable up to the expiry date of the kit in the broken, but tightly closed bag when stored at 2-8°C.

Precision

Intra-assay and inter-assay CVs < 30%

Sensitivity

The lowest detectable level (Lowest detection limit, LOD) that can be distinguished from the zero standard is 3 ng/mL
Functional sensitivity (Limit of quantification-LOQ): 10 ng/mL

General Description

Ipilimumab is a monoclonal antibody, more specifically a fully humanized IgG1 antibody produced in mammalian cell culture, to the cytotoxic T lymphocyte antigen-4 (CTLA-4) which activates antitumor immunity by inhibiting this major checkpoint. The cytotoxic T-lymphocyte antigen-4 (CTLA-4) is a cell surface molecule that plays an essential role in regulating the adaptative immune response. The activation of T-cells is tightly controlled by two main signals 1) initial binding of tumour-associated antigens on antigen presenting cells to T-cell receptors and 2) subsequent binding of B7 molecules on the antigen presenting cells to CD28 receptors on T cells. After T-cell activation, CTLA-4 will be upregulated and it will compete on the binding between CD28 and B7. The binding between CTLA-4 and B7 interrupts the stimulatory signal which in order blunts T-cell proliferation response. The action of ipilimumab produces an exacerbated autoimmunity. This is explained as the absence or presence of CTLA-4 can augment or suppress the immune system's T-cell response in fighting disease. Ipilimumab is designed to block the activity of CTLA-4, thereby sustaining a potent T-cell response against tumour cells.