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Creative Diagnostics provides Chromatin Immunoprecipitation (ChIP) Services for accurate profiling of protein and DNA interactions. We optimize antibodies and maintain rigorous protocols for efficient enrichment. The entire process from chromatin preparation to qPCR or ChIP sequencing is available for comprehensive analysis. Supports research in gene regulation, epigenetics and transcriptional control.
Diagenode Bioruptor® Pico / Plus
Promega Maxwell® RSC
Illumina NovaSeq 6000
Creative Diagnostics provides comprehensive Chromatin Immunoprecipitation (ChIP) Services for the study of protein-DNA interactions. ChIP is a valuable tool for epigenetic modification studies as well as transcriptional regulation studies. As a leader in the field of chromatin biology, we are well equipped to assist with the design of your ChIP assay strategy. Our services include transcription factor mapping, histone modification profiling, and chromatin structure analysis. Our robust platform includes optimized chromatin preparation, high-affinity antibody selection, and sensitive detection techniques for the best quality, reproducible results. We provide:
We offer ChIP Services to suit your needs of a fast and reliable ChIP analysis of Protein-DNA interactions. Chromatin Immunoprecipitation (ChIP) has been widely used for studying the association between protein and genomic DNA. The cross-linking of chromatin, lysis, and immunoprecipitation technologies are used to effectively immunoprecipitate the binding site of the target protein of interest and avoid the problems of non-specific binding and sample complexity. The service includes optimized sample preparation, specific antibody screening, strict immunoprecipitation, and highly sensitive downstream detection for the best data quality and most in-depth analysis. This technology has been successfully applied in the localization of transcription factors, the research of epigenetic modifications, the analysis of gene regulatory networks and the mechanism of disease. Based on the complete quality control system and rich experience in technical support, we ensure the specificity, reproducibility and stability of each experiment result. Our core services include:
Process samples and fix protein-DNA binding.
Lyze and shear chromatin.
Enrich target complexes using antibodies.
Purify immunoprecipitated DNA.
Perform qPCR or sequencing.
Provide data analysis and technical support.
The ChIP-seq peak signal plot displays the distribution of target transcription factor binding intensity near the transcription start site (TSS). The horizontal axis represents the relative position from the TSS (unit: bp), and the vertical axis represents the enrichment signal intensity. The main peak is concentrated in the TSS region, suggesting that this transcription factor may be directly involved in gene promoter regulation.
ChIP is primarily used to identify which proteins "shake hands" with which locations on the genome, helping you understand how genes are switched on and off.
No problem. We have optimized protocols that support small sample sizes, such as primary cells or precious tissues, while still obtaining reliable data.
Use validated, high-affinity, strong specificity antibodies. This will give better immunoprecipitation and more accurate data.
In a nutshell, ChIP-qPCR is good for validation of binding to specific genes, while ChIP-seq is used to comprehensively scan binding hotspots throughout the whole genome.
It usually takes about 3-4 weeks from receiving the samples to complete the experiment and basic data analysis. Time may vary according to the complexity of the project.
To use our ChIP services, please contact Creative Diagnostics, your expert immunology service provider. From research-scale ChIP services to industrial scale-up, our team is ready to support your R&D innovation.
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