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Creative Diagnostics offers Single-domain Antibodies (small, stable, antibody fragments with superior tissue penetration and capacity to bind hidden or complex epitopes). They are derived from camelid heavy-chain antibodies, offering high specificity and affinity with robust stability in harsh environments. Using advanced antigen design and optimized screening, we ensure single-domain antibodies that reliably recognize native protein conformations with low background. Ideal for diagnostics, imaging, and biosensing, our Single-domain Antibodies deliver sensitive and accurate detection of challenging targets across research and clinical applications.
Cytiva ÄKTA Pure 25 / ÄKTA Go
Sartorius Octet RED96e (BLI)
Eppendorf BioFlo® 320
Creative Diagnostics supports all stages of development for high-affinity, high-specificity single-domain antibodies, for both research and diagnostic or therapeutic applications. Our experience in immunology and protein engineering allows us to provide customer with a development plan to address the unique challenges involved with producing stable and functional single-domain antibodies. Our platform for producing single-domain antibodies is based on an optimized antigen design strategy, effective immunization, and screening and validation methods that provide reliable, high-performance single-domain antibodies with superior specificity and consistency. We provide:
The demand for high-affinity and highly specific antibody reagents and solutions is on the rise and our Single-domain Antibody Production Services are set up to take full advantage of the unique properties offered by single-domain antibodies. Their small size, stability and ability to bind to hidden or recessed epitopes make them the ideal choice for most difficult targets and complex biological matrices. Based on an efficient antigen design strategy as well as a highly optimized camelid hosts and high-throughput screening capabilities, which together guarantee the production of high quality and high performance antibodies. Every step of the process from antigen preparation, through immunization monitoring and final clone selection is tailored to provide the highest possible yield, affinity and specificity. The resulting antibodies are ideally suited for therapeutic candidate discovery, biomarker validation, molecular imaging and diagnostic assay development. The service is supported by a full quality system and is backed by a technical team with in depth expertise and an extensive track record that can consistently deliver single domain antibodies with high reproducibility, stability and function. Our core services include:
Evaluate antigen characteristics and determine antibody development strategies.
Immunize in animals such as alpacas and monitor antibody response levels.
Isolate immune cells and construct VHH phage display libraries.
Screen high-affinity antibodies and verify binding activity and specificity.
Recombinantly express and purify antibodies, and provide complete quality control and technical support.
In this case, in order to verify the affinity of the developed single-domain antibody, Creative Diagnostics used BLI (Bio-Layer Interferometry) technology for binding kinetics testing. The figure below shows a typical BLI sensogram, where the horizontal axis is time (seconds) and the vertical axis is the binding response signal (nm offset). The blue curve intuitively shows the antibody's quick elevation in the binding phase and slow decay in the dissociation phase, and the gray dotted line in the middle represents the demarcation between the two phases. The "fast binding, slow dissociation" property indicates that the antibody has high affinity and good stability to the target antigen.
Single-domain antibodies are small in size and simple in structure. They not only have strong penetration, but also can bind to hidden epitopes that are difficult for traditional antibodies to recognize. They are also thermally stable and suitable for a variety of extreme experimental environments.
We mainly use alpacas (or camels) for immunization. These animals can produce natural heavy chain antibodies (VHH) and are an ideal source of single-domain antibodies.
Yes. We can enhance immunogenicity by coupling carriers or designing special immunization strategies. Even small molecules or short peptides can successfully develop high-quality single-domain antibodies.
You will receive expression plasmids, purified antibody proteins, sequencing data, affinity test reports, and complete experimental process documentation to ensure reusability and subsequent development.
Very wide range of uses! Including therapeutic antibody screening, targeted drug delivery, in vivo imaging, immunodiagnosis, tissue staining, and even the construction of bispecific antibodies or CAR-T systems.
To use our single-domain antibody production services, please contact Creative Diagnostics, your expert immunology service provider. From research-scale single-domain antibody production to industrial scale-up, our team is ready to support your R&D innovation.
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