Shipped at room temperature and it should be stored at room temp. DO NOT FREEZE. Stable for 6 months.
Recommended Usage
Suggested Usage: Adsorption of most proteins can be achieved by mixing equal volume of the antigen (preferably in saline) with an equal volume of the adjuvant to form an emulsion. (Ovalbumin/IFA (1:1, v/v was tested). Protein left in the supernatant can be measured by protein assay or specific ELISA. Dosage: Mouse/Rat/G.Pig (50-200 ul gel; IM/SC)Rabbit (250 ul; SC/IM).
Description
Incomplete Freund's Adjuvant (IFA) consists of heat-killed Mycobacterium tuberculosis in a water-in-oil emulsion. Additionally, IFA has ligands for TLR2, TLR4, and TLR9. Injection of antigen in IFA induces a Th1-dominated response. Freund's incomplete adjuvant lacks the mycobacterium found in Complete Freund's Adjuvant so it minimizes the side-effects. For this reason, Incomplete Freund's Adjuvant is used for the boost injections IFA is being used as an adjuvant in 42 vaccines currently. pH: 6.5 Binding capacity: 1 to 10 µg of endotoxin-free ovalbumin at Ph 7.0. Note: Protein binding will vary with protein concentration, buffer and incubation conditions.
Format
Provided as a ready-to-use ; cell suspension, sterile solution. Clear pale yellow liquid
Online Inquiry
My Review for Incomplete Freunds Adjuvant (IFA, Vaccine adjuvant)
Creative Diagnostics products are for RESEARCH USE ONLY, please make sure your review is research based.
Required fields are marked with *
Terms and conditions:
We will select high-quality review customers and offer a $30 coupon for your next purchase.
All product reviews must be submitted in the English language.
Creative Diagnostics will not share any personal information of applicants, and all information will be treated with strict confidentiality and will not be sold or disclosed to a third party.
