Anti-Human IKK beta monoclonal antibody

Western blot analysis of IKBKB was performed with 10 µg of A549 cells transfected with Transfection Reagent alone (Lane 1), 100nM Non-Targeting control siRNA (Lane 2), or 100nM siRNA against IKBKB (Lane 3). Proteins were resolved using a NuPAGE® Novex 4-12% Bis-Tris Gel , XCell SureLock™ Electrophoresis System , and a protein size ladder. Proteins were wet transferred to a Pierce Nitrocellulose Membrane OR Pierce PVDF Membrane and blocked with Pierce Starting Block T20 (PBS) Blocking Buffer for 1 hour at room temperature. IKBKB was detected at ~ 87 kDa using IKBKB Rabbit monoclonal antibody diluted in Pierce Starting Block T20 (PBS) Blocking Buffer 4°C overnight on a rocking platform. Pierce Goat Anti-Rabbit HRP-Conjugated Antibodies at a 1:2500 dilution were used and chemiluminescent detection was performed using Pierce Supersignal West Dura Maximum Sensitivity Substrate. Relative density of the bands normalized to GAPDH (36 kDa). IKBKB Antibody confirms silencing of IKBKB expression.