Materials and Reagents

IgM Purification Reagents

IgM monoclonal antibodies have become increasingly favorable for their use as therapeutic tools, while remaining popular for their research and diagnostic applications. Protein A and G chromatography continues to lead the life science industries for the purification of IgG, but these methods have proven unsuitable for the purification of IgM antibodies.

Creative Diagnostics offers a novel resin with the lead product performing exceptionally well in purification of many IgM species products, including human and mouse. The product is certified for 90% adsorption and 85% yield for human IgM supernatant purification. In addition to increased yields and purity levels, Magic™ Human IgM Purification Resin address many of the shortfalls related to standard affinity ligands including challenges with proteolysis and repeated sanitization associated with other common purification methodologies.

Our products are available in kit form and loose resin; 1 and 5 ml columns are also available.

Products

Cat No. Product Name Size Manual Price
CDN-RE1 Magic™ Human IgM Purification Resin 5 mL PDF INQUIRY
CDN-RE2 Magic™ Mouse IgM Purification Resin 5 mL PDF INQUIRY
CDN-HPC Magic™ Human IgM Purification Column 1 mL/5 mL PDF INQUIRY
CDN-MMC Magic™ Mouse IgM Purification Column 1 mL/5 mL PDF INQUIRY
CDN-HMK1 Magic™ Human IgM Purification Kit 10 spin columns kit PDF INQUIRY
CDN-MMK2 Magic™ Mouse IgM Purification Kit 10 spin columns kit PDF INQUIRY

Performance

IgM Purification Reagents Figure 1: Statistical analysis of all three human IgM purification resins in terms of % adsorption, % yield, and binding capacity; averages were based on calculations from each resin being tested in triplicate. (a) Bars represent the average percentages (±95% confidence interval) of human IgM adsorption and yield for each purification resin type. (b) Bars represent the average static binding capacity for human IgM (±95% confidence interval) for each purification resin type.

IgM Purification Reagents Figure 2: Coomassie-stained SDS-PAGE in reducing conditions; equivalent volumes of the flowthrough and elute fractions were analyzed. 1.7 µg of human IgM standard was loaded in the gel. The sample load was run at equivalent volume to the flowthrough fractions. Arrows indicate the position of heavy-chain (68 kDa) and light-chain (26 kDa) human IgM.

IgM Purification Reagents Figure 3: Analytical SEC-HPLC profiles of flowthrough and elute fractions; column: MabPac SEC-1 (30 cm); mobile phase: PBS; flow rate: 0.15 mL/min; wavelength: 280 nm; injection: 10 µL. (a) Flowthrough fraction analysis, including the sample load (starting material) and the human IgM reference standard (2.2 µg); (b) elution fraction analysis, including the human IgM reference standard (2.2 µg).

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