SecADC™ Anti-Mouse IgG Fc polyclonal antibody [CL-MMAE]

Antibody Isotype

IgG

Species Reactivity

Mouse

Conjugate

Application Notes

Used in cell-based cytotoxic assay.
Anti-Mouse IgG Fc polyclonal antibody [CL-MMAE] is a secondary antibody-drug conjugates for pre-screening antibodies with a Mouse IgG Fc moiety or recombinant Mouse IgG Fc fusion proteins to determine their cytotoxicity as MMAE bioconjugates. It alone displays no obvious toxicity against multiple cell lines at 1ug/ml (6.6 nM) or lower concentration. When applied in combination with tumor specific monoclonal antibodies or recombinant Fc fusion proteins, it can help determine the cytotoxic potential for these antibodies or proteins against target cell lines.

General Notes

SecADC™ Anti-Mouse IgG Fc polyclonal antibody [CL-MMAE] can be used in cell-based cytotoxicity assays to pre-screening monoclonal antibodies as ADC candidates against tumor cells. This product is anti-Mouse IgG Fc specific antibody conjugated to monomethyl auristatin E (MMAE) with a cleavable linker. The antibody portion is a polyclonal antibody which is specific to the Fc region of Mouse IgGs. Monomethyl auristatin E (MMAE) is a cytotoxic small molecule which inhibits cell division by blocking the polymerization of tubulin. The cleavable linker connecting MMAE to the antibody is stable in extracellular fluid, but is cleaved by cathepsin in endosome once the conjugate has entered a cell via endocytosis.

Alternative Names

Ig gamma 1 chain C region; Immunoglobin heavy constant gamma 1; Immunoglobulin G; IgG; IgG heavy chain; Immunoglobulin G heavy chain; IGHG1; IGHG2; IGHG3; IGHG4;

Pathway

IL4-mediated signaling events;