Human IgG1 Isotype Control [MMAE]

Antibody Isotype

IgG1, κ

Species Reactivity

Chicken

Immunogen

HEL (Hen Egg Lysozyme)

Conjugate

Application Notes

ELISA, FC, IF, IHC, in vivo, IP, WB
Each laboratory should determine an optimum working titer for use in its particular application.
Other applications have not been tested but use in such assays should not necessarily be excluded.

General Notes

This product is a control ADC for any IgG type antibody labeled with MMAE. It has an average of 4 MMAE loaded on each antibody and can be used for accessing the effect of MMAE alone (non-specific toxicity). The product uses a very low endotoxin level antibody as a starting material (≤0.5 EU/mg by the LAL method). The preparation and purification are conducted under pyrogen free environment. The final product is assayed for endotoxin to make sure it is suitable for cell culture and animal studies. The loading is confirmed by various methods including UV, HPLC, and difference MALDI-TOF MS.

Alternative Names

Ig gamma 1 chain C region; Immunoglobin heavy constant gamma 1; Immunoglobulin G; IgG; IgG heavy chain; Immunoglobulin G heavy chain; IGHG1; IGHG2; IGHG3; IGHG4;

Pathway

IL4-mediated signaling events;