Product Overview
A DNA sequence encoding the N-terminal fragment (Met1-Arg362) of the influenza B hemagglutinin (influenza B virus (B/Brisbane/60/2008)) (ACN29383.1), termed as HA1, was fused with a polyhistidine tag at the C-terminus.
Molecular Weight
The secreted recombinant HA1 subunit of influenza A H5N1 HA (influenza B virus (B/Brisbane/60/2008)) comprises 358 amino acids and has a predicted molecular mass of 39.1 kDa. As a result of glycosylation, it migrates as an approximately 66 kDa band in SDS-PAGE under reducing conditions.
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
> 92 % as determined by SDS-PAGE
Format
Lyophilized from sterile PBS, pH 7.41. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Storage
Samples are stable for up to twelve months from date of receipt at -70°CStore it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Keywords
Influenzavirus B; Influenza B virus; IBV;IBV HA1; HA1
Citations
Publication ()
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COA
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