Magic™ Rabbit Anti-Human XRCC5 Monoclonal antibody, clone 351412B2

Antibody Isotype

IgG

Clone

351412B2

Species Reactivity

Human

Immunogen

XRCC5 fusion protein

Conjugate

Application Notes

We recommend the following antibodies for sandwich immunoassay (Capture - Detection):
DMAB-CPT1001 - DMAB-CPT1002

General Notes

This product (Catalog No. DMAB-CPT1002) is a rabbit-derived monoclonal antibody raised against human XRCC5. It has been affinity purified by Protein A. There are at least two pathways for eukaryotes to repair DNA double-strand breaks: homologous recombination and nonhomologous end joining(NHEJ). The core NHEJ machinery includes XRCC4, DNA ligase IV and the DNA-dependent protein kinase complex, which consists of the DNA end-binding XRCC5/XRCC6 heterodimer and the catalytic subunit PRKDC. The heterdimer of XRCC5/XRCC6 enhanced teh affinity of the catalytic subunit PRKDC to DNA by 100-fold. Once the XRCC5/6 dimer association with NAA15, it can bind to the osteocalcin promoter and activate osteocalcin expression. The XRCC5/6 dimer acts as a negative regulator of transcription when together with APEX1. Some publised papers indicated that the MW of XRCC5 is 86kDa, while more papers suggested that XRCC5 is a 80kDa protein, as it was firstly introducted in publication.
DMAB-CPT1002 is suitable for use in CBA(Det), ELISA(Det) applications.
DMAB-CPT1002 is supplied as a liquid formulation in PBS only. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Alternative Names

KU80; CTCBF; CTC85; CTC box-binding factor 85 kDa subunit; ATP-dependent DNA helicase II 80 kDa subunit; X-ray repair complementing defective repair in Chinese hamster cells 5

Entrez Gene ID

UniProt ID