Sample
serum, plasma, cell culture supernatants, tissue homogenate
Detection Method
Competitive ELISA
Intended Use
For quantitative detection of Human WNT3A (Protein Wnt-3a) in serum, plasma, tissue homogenates and other biological fluids.
Contents of Kit
1. 96-well strip plate (Dismountable), 1 plate
2. Lyophilized Standard, 2 vials
3. Sample/Standard dilution buffer, 20 mL
4. Biotin-detection antibody (Concentrated), 60 uL
5. Antibody dilution buffer, 10 mL
6. HRP-Streptavidin Conjugate(SABC), 120 uL
7. SABC dilution buffer, 10 mL
8. TMB substrate, 10 mL
9. Stop solution, 10 mL
10. Wash buffer (25X), 30 mL
11. Plate Sealer, 5 pieces
12. Product Manual, 1 copy
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Precision
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Detection Range
1.56-100 ng/mL
Citations
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| Product Name |
Cat. No. |
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Datasheet |
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Add to Basket |
Lee, YN; Gao, Y; et al. Differential mediation of the Wnt canonical pathway by mammalian Dishevelleds-1,-2, and-3. CELLULAR SIGNALLING 20:443-452(2008).
Binnerts, ME; Tomasevic, N; et al. The First Propeller Domain of LRP6 Regulates Sensitivity to DKK1. MOLECULAR BIOLOGY OF THE CELL 20:3552-3560(2009).
COA
Certificate of Analysis
