General Notes
This product (Catalog No. CABT-L1679W) is a mouse-derived monoclonal antibody. This antibody exhibits cross-reactivity with from mouse and rat. It has been affinity purified by Protein A. VDAC2, or voltage-dependent anion channel 2, is a pore-forming protein encoded by the VDAC2 gene on human chromosome 10q22.2 that primarily resides in the outer mitochondrial membrane, functioning as a non-selective voltage-gated ion channel to facilitate the diffusion of anions, cations, metabolites (such as ATP and NADH), and small hydrophilic molecules up to 5-6 kDa between the cytosol and the mitochondrial intermembrane space. Structurally, VDAC2 forms a transmembrane β-barrel composed of 19 antiparallel β-strands, approximately 2.6 nm in diameter, with a flexible N-terminal α-helical domain that lies within the pore and modulates channel activity; this architecture, resolved through crystallographic studies of zebrafish VDAC2 (sharing >90% sequence identity with the human isoform), exhibits conformational heterogeneity and includes mammal-specific features like an 11-12 amino acid N-terminal extension and higher cysteine content (9 residues) potentially involved in redox regulation and protein-lipid interactions. At low membrane potentials (<30 mV), the channel remains predominantly open with a conductance of about 4.5 nS in 1 M KCl and weak anion selectivity, but it undergoes voltage-dependent gating at higher potentials, transitioning to a cation-selective, half-conductance state. Beyond solute transport, VDAC2 plays essential non-redundant roles in cellular processes, including mediating calcium transfer from the sarcoplasmic reticulum to mitochondria in cardiomyocytes via interaction with ryanodine receptor 2 (RyR2) to support rhythmic contractions, facilitating cholesterol import for steroidogenesis through binding steroidogenic acute regulatory protein (StAR), and regulating apoptosis by recruiting and inhibiting BCL2-antagonist/killer 1 (Bak) under resting conditions while promoting Bax oligomerization and outer membrane permeabilization upon death stimuli like truncated Bid. It also modulates mitochondrial permeability transition pore (mPTP) formation via glycogen synthase kinase-3β (GSK3β) translocation, suppresses autophagy by stabilizing the BECN1-BCL2L1 complex in ovarian folliculogenesis, enhances endothelial nitric oxide synthase (eNOS) activity in pulmonary endothelium, and contributes to ciliogenesis and sperm motility. Unlike VDAC1, which dominates metabolite flux and anti-apoptotic interactions, and VDAC3, which focuses on reactive oxygen species control, VDAC2's unique "open cup" structure (formed by residues Thr168 and Asp170) enables exclusive Bak binding and pro-apoptotic functions, with whole-body knockout being embryonically lethal in mice while heart-specific deletion causes cardiomyopathy.
CABT-L1679W is suitable for use in WB 1 (500-2000) application.
CABT-L1679W is supplied as a liquid formulation in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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