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The Human Triiodothyronine (T3) ELISA Kit is a sophisticated in vitro diagnostic tool designed for the precise quantification of total triiodothyronine in human serum and plasma. Utilizing a competitive enzyme-linked immunosorbent assay (ELISA) format, this kit provides clinicians and researchers with a reliable method for assessing thyroid function and diagnosing thyroid disorders. With its optimized protocol and ready-to-use components, the kit delivers excellent reproducibility and sensitivity, making it an essential asset for clinical laboratories, research institutions, and healthcare facilities involved in endocrine evaluation and metabolic studies.
Triiodothyronine (T3) is the biologically active thyroid hormone that plays a crucial role in regulating metabolic processes throughout the body. While produced in smaller quantities than thyroxine (T4), T3 possesses significantly greater biological activity and serves as the primary mediator of thyroid hormone effects on target tissues. The majority of circulating T3 is bound to carrier proteins, with only the free fraction being biologically active. Measurement of total T3 levels provides valuable clinical information for diagnosing various thyroid disorders, including hyperthyroidism, hypothyroidism, and thyroid hormone resistance syndromes.
Figure 1. Thyroid hormones.
The significance of T3 quantification extends beyond routine thyroid function assessment. In clinical practice, T3 measurements are particularly important for diagnosing T3 toxicosis, monitoring thyroid cancer patients, and evaluating the effectiveness of thyroid replacement therapy. Research applications include investigating metabolic disorders, studying the hypothalamic-pituitary-thyroid axis, and exploring the role of thyroid hormones in development and cellular function. This ELISA kit employs a competitive binding principle that ensures accurate detection of T3 across clinically relevant concentrations, providing reliable results for both diagnostic and research purposes.
Sample Compatibility
Sensitivity Range
Main Uses
Product Specifications
Intended Use & Research Applications
Main Features of the Product
| Parameter | Details |
| Product name | Human T3 ELISA Kit |
| Sample | serum |
| Intended Use | Human T3 ELISA Kitis an Enzyme Immunoassay (EIA) for the quantitative determination of triiodothyronine (T3) in human serum. For professional use only. |
| Storage | Store components of the kit at 2-8°C upon arrival up to the expiration date. For more detailed information, please download the following document on our website. |
Unlike generic ELISA kits, this kit is tailored to real-world endocrine research needs:
Validated for EDTA/Heparin plasma (the most common plasma types in thyroid studies) and excludes citrate plasma (which binds T3 and causes false lows)—a detail many competing kits overlook.
The sample dilution buffer contains thyroid hormone stabilizers (e.g., bovine serum albumin) to prevent T3 degradation during assay, critical for samples stored for > 24 hours.
Exceptional Sensitivity
This kit is engineered for high sensitivity, capable of accurately quantifying the low levels of progesterone present in the follicular phase of the menstrual cycle and in men. This is essential for obtaining clinically meaningful data across all physiological states.
Time-Efficient Workflow
The competitive ELISA format allows for a rapid and simplified procedure. With pre-coated plates and optimized reagents, the entire assay can be completed in approximately 90 minutes, facilitating timely decision-making in both research and clinical settings.
High Specificity
The antibody used in this kit exhibits high affinity and specificity for progesterone, minimizing interference from other structurally similar steroid hormones. This ensures the accuracy and reliability of results, which is paramount for fertility assessment and pregnancy monitoring.
The Human Triiodothyronine (T3) ELISA Kit represents a reliable and accurate solution for the quantification of total T3 in clinical and research settings. With its exceptional analytical performance, user-friendly design, and robust reproducibility, this kit provides healthcare professionals and researchers with a valuable tool for assessing thyroid function and advancing our understanding of thyroid physiology. The combination of clinical utility and research flexibility makes this kit suitable for various applications, from routine diagnostic testing to investigative studies in thyroidology and metabolic research.
A1: Elevated T3 levels are commonly seen in hyperthyroid conditions such as Graves' disease, toxic nodular goiter, and thyroiditis. Decreased T3 levels may occur in hypothyroidism, non-thyroidal illness (euthyroid sick syndrome), malnutrition, and during certain medications. T3 measurement is particularly valuable in diagnosing T3 toxicosis, where T3 is elevated while T4 remains normal.
A2: Serum samples should be collected using standard venipuncture techniques. Samples are stable for 24 hours at 2-8°C or for longer periods when stored at -20°C. Avoid repeated freeze-thaw cycles, as this may affect analyte stability and assay performance. Hemolyzed or lipemic samples may interfere with assay results and should be avoided.
A3: This T3 ELISA provides specific quantification of total triiodothyronine levels, complementing other thyroid tests including TSH, T4, and free T3 measurements. The competitive ELISA format offers excellent sensitivity and specificity for T3 detection, making it suitable for both routine clinical use and research applications. Results should be interpreted in the context of other thyroid function tests and clinical findings.
A4: Total T3 measures both protein-bound and free fractions of triiodothyronine, representing the overall T3 pool in circulation. Free T3 measures the biologically active fraction not bound to carrier proteins. While free T3 may better reflect biological activity, total T3 measurement remains clinically valuable and is affected by conditions that alter thyroid hormone binding proteins.
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