Human SP-10/ACRV1 Protein [His]

Product Overview

A DNA sequence encoding the mature form of human ACRV1 isoform 1 (P26436-1) (Gln 22-Ile 265) was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.

Target

Human SP-10

Nature

Recombinant

Predicted N terminal

Gln 22

Tag/Conjugate

His

Molecular Weight

The recombinant human ACRV1 consists of 255 amino acids and predictes a molecular mass of 27.3 kDa. In SDS-PAGE under reducing conditions, rhACRV1 migrates as an approximately 38 kDa band due to glycosylation.

Endotoxin

< 1.0 EU per ug of the protein as determined by the LAL method

Alternative Names

ACRV1; acrosomal vesicle protein 1; SP-10; SPACA2; D11S4365; acrosomal protein SP-10; sperm protein 10

Purity

> 90 % as determined by SDS-PAGE

Format

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4

Preservative

None

Storage

Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Introduction

Acrosomal protein SP-1, also known as Acrosomal vesicle protein 1 and ACRV1, is a testis-specific, differentiation antigen, that arises within the acrosomal vesicle during spermatogenesis, and is associated with the acrosomal membranes and matrix of mature sperm. Regulation of cell type-specific gene transcription is central to cellular differentiation and development. During spermatogenesis, a number of testis-specific genes are expressed in a precise spatiotemporal order. The longest transcript of ACRV1 / SP-1 is the most abundant, comprising 53-72% of the total acrosomal vesicle protein 1 messages; the second largest transcript comprises 15-32%; the third and the fourth largest transcripts account for 3.4-8.3% and 8.7-12.5%, respectively; and the remaining transcripts combined account for < 1% of the total acrosomal vesicle protein 1 message. ACRV1 / SP-1 is a testis-specific acrosomal protein that has been detected in several species including humans. It may be involved in sperm-zona binding or penetration, and it is a potential contraceptive vaccine immunogen for humans. ACRV1 / SP-1 may be involved in sperm-zona binding or penetration. It is also a intra-acrosomal protein that is considered to be a vaccine candidate for immunocontraception.

Keywords

ACRV1; acrosomal vesicle protein 1; SP-10; SPACA2; D11S4365; acrosomal protein SP-10; sperm protein 10