Human SARS-CoV-2 (Covid-19) Spike Protein S2 Antigen ELISA Kit

Sample

Human serum and plasma and cell culture supernatant

Species Reactivity

Human

Detection Method

sELISA

Intended Use

Enzyme Immunoassay for the Quantitative Antigen Determination of SARS-CoV-2 (Covid-19) Spike Protein S2 in human serum and plasma and cell culture supernatant.

Contents of Kit

1. SARS-CoV-2 (Covid-19) spike protein S2 Antibody Coated Microtiter Plate (12 x 8 wells) - 1 no
2. Human SARS-CoV-2 spike protein S2 Standards (0.5 ml) - 0, 15.6, 31.25, 125, 250, 500 ng/ml
3. SARS-CoV-2 (Covid-19) Antibody:HRP Conjugate - 12 ml
4. (5X) Assay Diluent - 25 ml
5. (20X) Wash Buffer - 25 ml
6. TMB Substrate - 12 ml
7. Stop Solution - 12 ml
8. Instruction Manual
9. Plate Sealer

Storage

1. Store main kit components at 2-8°C.
2. Before using, bring all components to room temperature (18-25°C). Upon assay completion return all components to appropriate storage conditions.
3. The Substrate is light-sensitive and should be protected from direct sunlight or UV sources.

Precision

Intra-Assay
Three samples of known concentration were tested five times on one plate to assess intra-assay precision.
Inter-Assay:
Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Detection Range

0 ng/ml - 500 ng/ml

Detection Limit

It is defined as the lowest detectable concentration corresponding to a signal of Mean of '0' standard plus 2* SD. 10 replicates of '0' standards were evaluated and the LOD was found to be less than 6.8 ng/ml.

Sensitivity

The capture antibodies used in the kit are specific against 2019-nCoV Coronavirus spike and has 100% crossreactivity with SARS-CoV Spike S2 Protein and some cross-reactivity with SARS-CoV-2 Spike S1+S2 ECD Protein, SARS-CoV Spike S1+S2 ECD Protein, MERS-CoV Spike S1+S2 ECD Protein.
The kit has no cross-reactivity with MERS-CoV Spike Spike S2, SARS-CoV-2 (2019-nCoV) Spike S1, SARSCoV-2 (2019-nCoV) Spike RBD, HCoV-OC43-Spike S1+S2 Protein, HCoV-229E-Spike S1+S2 Protein, HCoVHKU1 (isolate N5)Spike S1+S2 Protein, HCoV-NL63-Spike S1+S2 Protein.

General Description

The ELISA kit is used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody. Double antibodies are used in this kit.
The spike protein (S-protein) of the SARS-CoV-2 virus is the common target for neutralizing antibodies and vaccines. Spike protein contains two subunits, S1 and S2. S1 contains a receptor binding domain (RBD), which is responsible for recognizing and binding with the cell surface receptor. S2 subunit contains other basic elements needed for the membrane fusion. SARS-CoV-2 (2019-nCoV) can infect the human respiratory epithelial cells through interaction with huamn ACE2. Indeed, the recombinant Spike protein can bind with recombinant ACE2 protein.

Standard Curve