Magic™ Rabbit Anti-Human RHOBTB1 Monoclonal antibody, clone 351364H21

Antibody Isotype

IgG

Clone

351364H21

Species Reactivity

Human

Immunogen

RHOBTB1 fusion protein

Conjugate

Application Notes

We recommend the following antibodies for sandwich immunoassay (Capture - Detection):
DMAB-CPT999 - DMAB-CPT1000
Cytometric Bead Array: 2.5-80 ng/mL

General Notes

This product (Catalog No. DMAB-CPT999) is a rabbit-derived monoclonal antibody raised against human RHOBTB1. It has been affinity purified by Protein A. Rho-related BTB domain containing protein 1 (RhoBTB1) is also named as KIAA0740. RhoBTB1 is an atypical Rho GTPase with two BTB domains in addition to its Rho domain. RhoBTB1 is a substrate for ROCK1, and mutation of putative phosphorylation sites reduces its association with Cullin3, a scaffold for ubiquitin ligases (PMID: 31431478). RHOBTB1 is a novel PPARγ gene target in vascular smooth muscle cells that mediates the protective effect of PPARγ by serving as a substrate adaptor between the Cullin-3 RING ubiquitin ligase and phosphodiesterase 5, thus restraining its activity through ubiquitination and proteasomal degradation.
DMAB-CPT999 is suitable for use in Cytometric Bead Array (2.5-80 ng/mL) application.
DMAB-CPT999 is supplied as a liquid formulation in PBS only. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Alternative Names

KIAA0740; RHOBTB1; Rho-related BTB domain containing 1

Entrez Gene ID

UniProt ID