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The Human Resistin ELISA Development Kit is a robust and sensitive tool designed for the quantitative detection of human Resistin (RETN) in various biological samples, including serum, plasma, and cell culture supernatants. Utilizing a sophisticated sandwich enzyme-linked immunosorbent assay (ELISA) format, this kit provides researchers with a reliable method to obtain precise data crucial for studying the role of this adipokine in insulin resistance, obesity, and related metabolic disorders. Its optimized protocol ensures high specificity and reproducibility, making it an indispensable asset for basic research, drug discovery, and biomarker evaluation.
Resistin, an adipokine predominantly secreted by adipose tissue in humans, is a cysteine-rich protein that has been identified as a critical molecular link between obesity and insulin resistance. It is implicated in impairing insulin sensitivity in target tissues, thereby playing a significant role in the pathogenesis of Type 2 Diabetes Mellitus (T2DM). Accurate measurement of Resistin levels is therefore paramount for understanding its physiological and pathophysiological functions, investigating metabolic syndrome mechanisms, and evaluating the efficacy of potential therapeutic interventions.
Figure 1. Resistin.
Abnormal resistin expression is a hallmark of multiple metabolic disorders:
Traditional resistin detection methods face critical limitations: Western Blot (WB) is semi-quantitative, low-throughput, and requires large sample volumes. Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) demands expensive equipment and specialized training. Generic ELISA kits often cross-react with other adipokines (e.g., adiponectin, leptin) or fail to detect low resistin levels in lean individuals. The human Resistin LISA kit addresses these gaps by offering a quantitative, high-specificity, cost-effective alternative—ideal for metabolic research teams of all sizes.
Sample Compatibility
Sensitivity Range
Main Uses
Product Specifications
Intended Use & Research Applications
Key Features of the Product
Performance
| Parameter | Details |
| Product name | Human Resistin ELISA Development Kit |
| Sample | Human |
| Intended Use | Human Resistin ELISA Development Kit contains the key components required for the quantitative measurement of natural and/or recombinant Resistin in a sandwich ELISA format. Using the ELISA protocol described below, this kit provides sufficient reagents to assay Resistin in approximately 1500 ELISA plate wells. |
| General Description | Resistin (RETN) is a peptide hormone secreted by adipocytes with potential roles in insulin resistance and adipocyte differentiation. Resistin functions as a regulator of glucose homeostasis and a physiologic antagonist to hepatic insulin action. Resistin may be an important candidate mediator of obesity-induced insulin resistance. It may also represent a novel link between metabolic signals, inflammation, and atherosclerosis. |
| Storage | Store the kit reagents at 2-8°C. For more detailed information, please download the following document on our website. |
For researchers focused on insulin resistance and obesity, this kit addresses core pain points in resistin research:
Exceptional Sensitivity
This kit is engineered for high sensitivity, enabling the accurate quantification of low physiological levels of Resistin. This is particularly crucial for research applications involving subtle changes in Resistin concentration, ensuring that researchers can capture biologically significant data with confidence.
High Specificity
The antibodies used in this kit have been meticulously selected for high specificity to human Resistin, demonstrating minimal cross-reactivity with other cytokines and related proteins. This ensures that the measurements accurately reflect the true concentration of Resistin in complex sample.
Accurate Clinical Assessment
The antibody used in this kit exhibits high affinity and specificity for progesterone, minimizing interference from other structurally similar steroid hormones. This ensures the accuracy and reliability of results, which is paramount for fertility assessment and pregnancy monitoring.
The human resistin ELISA kit is an essential tool for studying insulin resistance, obesity, and metabolic disorders, meeting the urgent need for specific, sensitive, and histocompatibility resistin quantification. Its ultra-high specificity eliminates cross reactivity of adipokines, while its lipid clearance buffer and protease inhibitor can accurately detect obesity related samples, which is the main limitation of alternative tools. Compared with WB and LC-MS/MS, it provides excellent quantitative accuracy and cost-effectiveness, supporting large-scale cohort studies and dynamic drug monitoring. Supported by strict quality control, comprehensive technical support, and validation performance of different metabolic samples, this kit accelerates the understanding of the role of resistin metabolic diseases and the development of targeted therapies. For anyone studying insulin resistance, obesity, or T2D, this kit is the best choice.
A1: Resistin is a key biomarker that links obesity with insulin resistance and type 2 diabetes. Elevated serum resistin levels are associated with the HOMA-IR index and the pathogenesis of metabolic syndrome, making it a valuable biomarker for metabolic disease research.
A2: The sandwich ELISA format is typically used for larger proteins such as resistin, as it involves two specific antibodies binding to different epitopes on the target molecule. Compared to competitive ELISA formats, this design typically has higher specificity and sensitivity, making it suitable for detecting proteins in complex biological fluids.
A3: No. Due to the high specificity of the antibodies used, this kit is specifically designed for detecting human resistin. The cross reactivity with resistins from other species has not been guaranteed and requires user verification.
A4: Yes, this product provides comprehensive technical support. If you have any questions about the testing plan, troubleshooting, or data interpretation, you can contact the technical team.
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