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The Human Prolactin ELISA Kit is a sophisticated and highly specific in vitro diagnostic tool designed for the precise quantification of human prolactin (PRL) in serum, plasma, and other biological samples. Utilizing an advanced sandwich enzyme-linked immunosorbent assay (ELISA) format, this kit provides researchers and clinicians with a reliable method to obtain accurate data essential for evaluating pituitary function, investigating reproductive disorders, and monitoring various endocrine conditions. With its optimized protocol and ready-to-use components, the kit ensures excellent reproducibility and sensitivity, making it an indispensable asset for clinical laboratories and research institutions focused on endocrine and reproductive medicine.
Figure 1. Hormones In Pregnancy.
Prolactin is a polypeptide hormone primarily synthesized and secreted by the lactotroph cells in the anterior pituitary gland. While its most recognized function is the stimulation of milk production during lactation, prolactin plays multifaceted roles in various physiological processes, including reproduction, metabolism, immune regulation, and behavior. The measurement of prolactin levels holds significant clinical importance in the diagnosis and management of numerous medical conditions. Elevated prolactin levels (hyperprolactinemia) can indicate pituitary tumors, particularly prolactinomas, and are associated with reproductive dysfunctions such as infertility, galactorrhea, and hypogonadism in both men and women. Conversely, low prolactin levels may signal general pituitary insufficiency.
Figure 2. Prolactin
This ELISA kit employs a sandwich immunoassay principle that ensures high specificity and sensitivity. The microplate is pre-coated with a monoclonal antibody specific for human prolactin. During the assay, prolactin in the sample binds to these immobilized antibodies. After washing to remove unbound materials, an enzyme-linked polyclonal antibody specific for prolactin is added to form a sandwich complex. Following another washing step, a substrate solution is added, producing a colored reaction product whose intensity is directly proportional to the concentration of prolactin present in the sample. This robust methodology allows for the accurate detection of prolactin across clinically relevant concentrations.
Sample Compatibility
Sensitivity Range
Main Uses
Intended Use & Research Applications
Key Features & Product Specifications
Precision
Key Features of the Product
| Parameter | Details |
| Product name | Mouse/Rat Prolactin ELISA Kit |
| Sample | plasma |
| Intended Use | The Prolactin ELISA kit is used for the quantitative measurement of prolactin in a Mouse/ Rat plasma. |
| Storage | 1. Store the kit at 2-8°C. 2. Keep microwells sealed in a dry bag with desiccants. 3. The reagents are stable until expiration of the kit. |
Exceptional Analytical Performance
The kit demonstrates outstanding sensitivity with a detection limit below 1.0 ng/mL, enabling accurate quantification of prolactin across the entire physiological and pathological range. The excellent precision profile, ensures reliable and reproducible results essential for both clinical monitoring and research applications.
Streamlined, Time-Efficient Workflow
The competitive ELISA format allows for a rapid and simplified procedure. With pre-coated plates and optimized reagents, the entire assay can be completed in approximately 90 minutes, facilitating timely decision-making in both research and clinical settings, and enabling high-throughput screening.
Superior Specificity and Reliability
Utilizing highly specific monoclonal and polyclonal antibodies, the assay demonstrates minimal cross-reactivity with related pituitary hormones and other potentially interfering substances. This high specificity ensures accurate measurement of prolactin in various clinical conditions.
The Human Prolactin ELISA Kit represents a sophisticated and reliable solution for the accurate quantification of prolactin in clinical and research settings. With its exceptional analytical performance, user-friendly design, and robust reproducibility, this kit provides healthcare professionals and researchers with a valuable tool for investigating endocrine disorders and advancing our understanding of prolactin's diverse physiological roles. The comprehensive nature of this kit makes it suitable for various applications, from routine clinical diagnostics to cutting-edge research in reproductive medicine and endocrinology.
A1: Elevated prolactin levels (hyperprolactinemia) can result from various conditions including prolactin-secreting pituitary tumors (prolactinomas), medications that affect dopamine pathways, hypothyroidism, chronic renal failure, and physiological states such as pregnancy and lactation.
A2: Serum samples should be collected using standard venipuncture techniques. Samples are stable for 24 hours at 2-8°C or for longer periods when stored at -20°C. Avoid repeated freeze-thaw cycles, as this may affect analyte stability and assay performance.
A3: The hook effect, or high-dose hook effect, can occur in immunometric assays when extremely high analyte concentrations saturate both capture and detection antibodies, leading to falsely low results. This kit incorporates design features to minimize this effect, but samples with suspected very high prolactin concentrations should be tested at multiple dilutions.
A4: The kit primarily detects monomeric prolactin (23 kDa), which constitutes the majority of circulating prolactin and possesses the highest biological activity. While it may detect some larger molecular forms, the detection efficiency for macroprolactin (a complex of prolactin and IgG) may vary.
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