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The Human Progesterone ELISA Kit from Creative Diagnostics is a robust and highly specific tool designed for the quantitative detection of progesterone in human serum, plasma, and other biological fluids. Utilizing a sensitive and validated competitive enzyme-linked immunosorbent assay (ELISA) format, this kit provides researchers and clinicians with a reliable method to obtain precise data essential for assessing ovarian function, monitoring pregnancy, and evaluating endocrine health. Its optimized protocol ensures excellent reproducibility, making it an indispensable asset for research in reproductive medicine, obstetrics, gynecology, and endocrinology.
Progesterone is a critical steroid hormone produced primarily by the corpus luteum in the ovary during the second half of the menstrual cycle (luteal phase), and by the placenta during pregnancy. It plays a fundamental role in regulating the menstrual cycle, preparing and maintaining the endometrium for implantation, and supporting early pregnancy. Accurate measurement of progesterone levels is vital for evaluating ovulation, diagnosing luteal phase defects, assessing the risk of early pregnancy loss, and monitoring the health of a pregnancy. It is also crucial in assisted reproductive technology (ART) cycles to monitor endometrial receptivity and luteal phase support.
Figure 1. Female Hormone Lifecycle.
This ELISA kit operates on the competitive principle. Endogenous progesterone in the sample competes with a progesterone-enzyme conjugate (e.g., horseradish peroxidase, HRP) for binding sites on a limited amount of progesterone-specific antibody that is pre-coated onto the microplate. After an incubation and washing step to remove unbound materials, a substrate solution is added, producing a colorimetric signal. The intensity of this signal is inversely proportional to the concentration of progesterone in the sample, allowing for accurate quantification.
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Key Features of the Product
| Parameter | Details |
| Product name | Progesterone Human ELISA Kit |
| Sample | serum |
| Intended Use | For the quantitative measurement of Human progesterone concentrations in serum. |
| Storage | Store the kit reagents at 2-8°C. For more detailed information, please download the following document on our website. |
When compared to alternative progesterone detection methods or other ELISA kits on the market, the Human Progesterone ELISA Kit stands out for the following reasons:
Exceptional Sensitivity for Critical Low-Level Detection
This kit is engineered for high sensitivity, capable of accurately quantifying the low levels of progesterone present in the follicular phase of the menstrual cycle and in men. This is essential for obtaining clinically meaningful data across all physiological states.
Streamlined, Time-Efficient Workflow
The competitive ELISA format allows for a rapid and simplified procedure. With pre-coated plates and optimized reagents, the entire assay can be completed in approximately 90 minutes, facilitating timely decision-making in both research and clinical settings, and enabling high-throughput screening.
High Specificity for Accurate Clinical Assessment
The antibody used in this kit exhibits high affinity and specificity for progesterone, minimizing interference from other structurally similar steroid hormones. This ensures the accuracy and reliability of results, which is paramount for fertility assessment and pregnancy monitoring.
The Human Progesterone ELISA Kit is a specialized, high-performance tool tailored to the unique needs of reproductive health research. Its combination of high sensitivity, specificity, and user-friendliness addresses the limitations of traditional progesterone detection methods, while its strict quality control and robust performance ensure reliable data for basic research, clinical studies, and pharmaceutical R&D. Whether exploring the physiological role of progesterone, investigating reproductive disorders, or evaluating hormone-based therapies, this kit provides researchers with a cost-effective, efficient, and trustworthy solution. By simplifying complex detection processes and delivering accurate results, it contributes to advancing our understanding of reproductive health and improving outcomes in fertility and pregnancy-related research.
A1: Progesterone is a small molecule (steroid hormone) with a single antigenic epitope. This makes it impossible for two antibodies to bind simultaneously, as required in a sandwich ELISA format. The competitive ELISA is the established and most effective method for accurately quantifying small molecules like steroids.
A2: Progesterone levels are low (<1 ng/mL) during the follicular phase. After ovulation, levels rise significantly, typically exceeding 5 ng/mL during the mid-luteal phase, confirming that ovulation has occurred. Levels then drop if pregnancy does not occur.
A3: The kit should be stored at 2-8°C upon receipt. Under these conditions, all components are stable until the expiration date printed on the box. Avoid freezing the reagents unless specifically indicated in the manual.
A4: This kit is specifically validated for human progesterone. Cross-reactivity with progesterone from other species may vary and has not been validated. For animal studies, a species-specific ELISA kit is recommended.
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