The High-Sensitivity Human Oestradiol (E2) ELISA Kit is a state-of-the-art, rigorously validated research tool designed for the precise, ultra-sensitive quantification of human oestradiol (E2) in biological samples (e.g., serum, plasma, urine, follicular fluid, cell culture supernatants). As the primary endogenous oestrogen, E2 plays a pivotal role in regulating reproductive physiology (menstrual cycle, pregnancy, sexual development), bone metabolism, cardiovascular homeostasis, and mammary gland growth. Abnormal E2 levels are closely linked to reproductive disorders, hormone-dependent cancers, and metabolic diseases. This kit leverages advanced sandwich ELISA technology with high-affinity monoclonal antibodies, enabling detection of E2 at picogram-level concentrations—empowering researchers to unravel E2's molecular mechanisms in disease pathogenesis, validate biomarkers, and accelerate drug development for hormone-related disorders.
Oestradiol (E2) is the primary and most potent form of estrogen in the human body. It plays a fundamental role in regulating the menstrual cycle, supporting pregnancy, maintaining bone density, and influencing cardiovascular health. Accurate measurement of Oestradiol levels is vital for diagnosing and managing a wide array of conditions, including infertility, polycystic ovary syndrome (PCOS), menopausal disorders, and certain hormone-dependent cancers (e.g., breast cancer). Monitoring Oestradiol is also essential in assisted reproductive technology (ART) and hormone replacement therapy (HRT).
Figure 1. Oestradiol (E2).
This ELISA kit is based on the competitive principle. Endogenous Oestradiol in the sample competes with an Oestradiol-enzyme conjugate for binding to a limited amount of Oestradiol-specific antibody that is pre-coated onto the microplate. After a washing step to remove unbound materials, a substrate solution is added, producing a blue color. The reaction is stopped, and the resulting yellow color is measured spectrophotometrically. The intensity of the color is inversely proportional to the concentration of Oestradiol in the sample, allowing for accurate quantification.
Sample Compatibility
Sensitivity Range
Main Uses
Feature/Specification
Performance
Intended Use
Key Features of the Product
| Parameter | Details |
| Product name | Human Oestradiol ELISA Kit |
| Sample | serum, plasma |
| Intended Use | Human Oestradiol ELISA Kit is an Enzyme Immunoassay (EIA) for the quantitative determination of total Oestradiol (E2) in human serum or plasma. For professional use only. |
| Storage | Store the kit reagents at 2-8°C. Do not expose to direct sunlight. DO NOT FREEZE ANYOF THE REAGENTS as this will cause irreversible damage. For more detailed information, please download the following document on our website. |
Compared to alternative E2 detection methods and competing kits, this kit stands out for three core reasons:
Exceptional Sensitivity for Low-Level Detection
This kit is engineered for high sensitivity, capable of accurately quantifying the low levels of Oestradiol found in men, postmenopausal women, and during certain clinical scenarios. This is critical for obtaining meaningful data in a wide range of physiological conditions.
Streamlined, Time-Efficient Workflow
The competitive ELISA format often allows for a shorter total assay time compared to sandwich formats. With pre-coated plates and optimized reagents, the entire procedure can be completed in under two hours, significantly enhancing laboratory throughput and efficiency.
High Specificity in Complex Matrices
The antibody used in this kit exhibits high affinity and specificity for 17β-Oestradiol, minimizing interference from other structurally similar steroid hormones present in serum or plasma. This ensures the accuracy and reliability of results in complex biological samples.
The High-Sensitivity Human Oestradiol ELISA Kit is an indispensable tool for reproductive endocrine, oncology, and metabolic research, addressing the critical need for accurate, ultra-sensitive E2 quantification. Its ability to detect low-abundance samples, robust anti-interference capability, broad sample compatibility, and cost-effectiveness make it ideal for a wide range of applications—from exploring E2's role in menstrual cycle regulation to validating E2 as a cancer biomarker. By overcoming the limitations of traditional methods and competing kits, this kit empowers researchers to advance understanding of E2-related diseases and accelerate the development of targeted therapies—solidifying its role as a must-have asset for hormone research laboratories.
A1: Oestradiol is a small molecule (steroid hormone) and has only one antigenic epitope, making it too small for two antibodies to bind simultaneously as required in a sandwich ELISA. The competitive format is the standard and most effective method for quantifying small molecules like steroids.
A2: Samples with high concentrations should be diluted with the appropriate zero standard or sample dilution buffer provided in the kit. It is recommended to perform a pilot test to determine the optimal dilution factor to bring the sample reading within the linear range of the standard curve.
A3: If the sample's absorbance value exceeds that of the highest standard (100 pg/mL), it indicates the gastrin concentration is above the detection range. In this case, you should dilute the sample with the kit's provided sample dilution buffer (or blank serum) at a suitable ratio and re-test. When calculating the final concentration, multiply the measured value by the dilution factor. It is recommended to perform a pre-test with a small sample volume to determine the optimal dilution ratio and avoid over-dilution (which may reduce accuracy).
A4: Yes, Creative Diagnostics provides comprehensive technical support for all its products. You can contact their technical team with any questions regarding the assay protocol, troubleshooting, or data interpretation.
