Sample
serum, plasma, cell culture supernatants, tissue homogenate
Detection Method
Sandwich ELISA
Intended Use
For quantitative detection of Human MSH2 (DNA mismatch repair protein Msh2) in serum, plasma, tissue homogenates and other biological fluids.
Contents of Kit
1. 96-well strip plate (Dismountable), 1 plate
2. Lyophilized Standard, 2 vials
3. Sample/Standard dilution buffer, 20 mL
4. Biotin-detection antibody (Concentrated), 120 uL
5. Antibody dilution buffer, 10 mL
6. HRP-Streptavidin Conjugate(SABC), 120 uL
7. SABC dilution buffer, 10 mL
8. TMB substrate, 10 mL
9. Stop solution, 10 mL
10. Wash buffer (25X), 30 mL
11. Plate Sealer, 5 pieces
12. Product Manual, 1 copy
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Precision
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Detection Range
78-5000 pg/mL
Citations
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Cohn, DE; Frankel, WL; et al. Improved survival with an intact DNA mismatch repair system in endometrial cancer. OBSTETRICS AND GYNECOLOGY 108:1208-1215(2006).
Hirano, K; Yamashita, K; et al. Non-Hodgkin's lymphoma in a patient with probable hereditary nonpolyposis colon cancer - Report of a case and review of the literature. DISEASES OF THE COLON & RECTUM 45:273-279(2002).