Application: We recommend the following antibodies for sandwich immunoassay (Capture - Detection): DMAB-CPT487 - DMAB-CPT488
Summary
Specifications
Antibody Isotype
IgG
Clone
341217B5
Species Reactivity
Human; Mouse
Immunogen
MELK fusion protein
Conjugate
Unconjugated
Applications
Application Notes
We recommend the following antibodies for sandwich immunoassay (Capture - Detection): DMAB-CPT489 - DMAB-CPT490 Sandwich ELISA: 0.313-20 ng/mL
General Notes
The Magic™ Rabbit Anti-Human MELK Monoclonal Antibody (clone 341217B5, product code DMAB-CPT489) is a rabbit monoclonal antibody specifically directed against human MELK (Maternal Embryonic Leucine Zipper Kinase). MELK is a serine/threonine protein kinase involved in cell cycle regulation, proliferation, apoptosis, and stem cell maintenance, and has been implicated in tumor progression and cancer cell survival. This antibody is designed for the specific detection of MELK and is supplied in an unconjugated format suitable for immunoassay development. This product is validated for use in sandwich ELISA (capture format) as part of a matched antibody pair system. The recommended pairing is DMAB-CPT489 (capture) with DMAB-CPT490 (detection), enabling sensitive sandwich ELISA detection with a working range of 0.313–20 ng/mL. It is suitable for quantitative detection of MELK in biological samples and is validated for human and mouse cross-reactivity, supporting multi-species applications in ELISA-based immunodetection workflows. DMAB-CPT489 is a rabbit IgG monoclonal antibody purified using Protein A affinity chromatography and supplied as a liquid formulation in PBS only. Each vial contains 100 μg of purified antibody. The product is shipped with ice packs to ensure stability during transport. Upon receipt, it should be immediately stored at −80°C for long-term preservation.
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