Human IgG ELISA Kit

Name: Human IgG ELISA Kit
SKU: DEIA1480U
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum

Species Reactivity

Human

Detection Method

Sandwich-ELISA

Intended Use

This kit allows measurement of human IgG1-4 subclasses in serum of mice, rats, dogs, and monkeys.

Contents of Kit

No.	Components	Size	Storage Conditions
1	Anti Human IgG Coated 96-well Plate	12×8 wells	2-8°C
2	HRP Conjugate	11 ml	2-8°C
3	Human IgG Stock	1	2-8°C
4	20× Wash Solution	50 ml	2-8°C
5	Diluent	50 ml	2-8°C
6	TMB Reagent	11 ml	2-8°C
7	Stop Solution	11 ml	2-8°C

Storage

The test kit will remain stable for six months from the date of purchase provided that the components are stored at 4°C. The microtiter plate should be kept in a sealed bag with desiccant to minimize exposure to damp air.

Performance Characteristics

Serum from swiss-webster mice, sprague dawley rats, mongrel dogs, and cynomolgus monkeys was spiked with 100 or 1000 ng/ml of human IgG1. Samples were tested at dilutions of 10, 20, 40… to 1280-fold. Recovery values were 100%. No significant human IgG reactivity was found in unspiked serum from the above species.

General Description

Pharmacokinetic (PK) characterization of human IgG monoclonal antibodies requires measurement of human IgG in serum of species used in preclinical research.

Standard Curve

A typical standard curve with A450 values on the Y-axis against IgG concentrations on the X-axis is shown below. The curve is for illustration only and should not be used to calculate unknowns.