General Notes
This product (Catalog No. CABT-L1585W) is a rabbit-derived polyclonal antibody raised against human HCRTR1/2, targeting the amino acid region 321–425 of the protein. This antibody exhibits cross-reactivity with Human HCRTR1/2 from mouse and rat. It has been affinity purified by Protein A. Orexin receptors, also known as hypocretin receptors, are a pair of G protein-coupled receptors (GPCRs) that bind the neuropeptides orexin-A and orexin-B, which are produced by neurons in the lateral hypothalamus and play central roles in regulating arousal, sleep-wake cycles, feeding behavior, and reward processing. These receptors, designated OX1R and OX2R, were identified in 1998 following the discovery of the orexin peptides themselves, marking a significant advancement in understanding the neural basis of energy homeostasis and vigilance. Structurally, both OX1R and OX2R feature seven transmembrane domains typical of GPCRs, with the two subtypes sharing approximately 64% amino acid sequence identity in humans. OX1R exhibits selective high affinity for orexin-A (IC50 ≈ 20 nM), while OX2R binds both orexin-A and orexin-B with similar affinity (IC50 ≈ 36–38 nM), enabling differential signaling in various brain regions. Upon ligand binding, these receptors couple primarily to Gq/11 proteins, activating downstream pathways such as phospholipase C, intracellular calcium mobilization via TRPC channels, and mitogen-activated protein kinase (MAPK) signaling, which collectively mediate excitatory effects on target neurons. The receptors are widely expressed throughout the central nervous system, with OX1R predominantly in areas involved in reward and emotion like the locus coeruleus and ventral tegmental area, and OX2R more abundant in arousal-regulating nuclei such as the tuberomammillary nucleus and dorsal raphe. Physiologically, the orexin receptor system stabilizes wakefulness and prevents intrusions of rapid eye movement (REM) sleep, as evidenced by narcolepsy in humans and canines resulting from orexin neuron loss or OX2R mutations. Beyond sleep, they influence appetite stimulation—orexin-A promotes feeding via OX1R in the hypothalamus—and modulate reward-seeking behaviors, stress responses, and even cognitive functions like learning and memory, with recent studies highlighting OX1R's role in reducing opioid motivation and OX2R's involvement in chemotherapy-induced anorexia alleviation.
CABT-L1585W is suitable for use in Western blot (WB) (1:500–1:2,000), IHC-P (1:100–1:500), IF (immunofluorescence) (1:100–1:500) applications.
CABT-L1585W is supplied as a liquid formulation in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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