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The Human Gastrin ELISA Kit from Creative Diagnostics is a sophisticated and reliable tool designed for the quantitative detection of Gastrin in human serum, plasma, and other biological fluids. Utilizing a sensitive sandwich enzyme-linked immunosorbent assay (ELISA) format, this kit provides researchers with a robust method to obtain precise data crucial for studying gastrointestinal physiology and pathology. Its optimized protocol ensures high specificity and reproducibility, making it an indispensable asset for research in endocrinology, gastroenterology, and related fields.
Gastrin is primarily synthesized and secreted by G cells in the antrum of the stomach and duodenum. Its main physiological functions include stimulating parietal cells to secrete gastric acid, promoting the proliferation of gastric mucosal cells, and regulating the contraction of gastrointestinal smooth muscles. Abnormal levels of gastrin (either elevated or decreased) are closely associated with a range of digestive diseases: for example, hypergastrinemia is a hallmark of gastrinomas (neuroendocrine tumors) and atrophic gastritis, while hypogastrinemia may occur in cases of gastric resection or autoimmune gastritis.
Figure 1. Gastrin.
Traditional methods for gastrin detection, such as radioimmunoassay (RIA), have limitations including radioactive waste disposal, short reagent shelf life, and low throughput. The Human Gastrin ELISA Kit addresses these drawbacks by combining the specificity of antibody-antigen binding with the efficiency of enzyme catalysis. It eliminates radioactive risks, extends reagent stability, and enables high-throughput testing (compatible with 96-well microplates), making it a preferred choice for both research and clinical laboratories seeking accurate, safe, and efficient gastrin quantification.
Sample Compatibility
Sensitivity Range
Main Uses
Feature/Specification
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Intended Use
Key Features of the Product
| Parameter | Details |
| Product name | Human Gastrin ELISA Kit |
| Sample | Serum, plasma, cell culture supernatant and other biological samples. |
| Intended Use | In vitro quantitative determination of GAST concentrations in serum, plasma, cell culture supernatant and other biological samples |
| General Description | Gastrin is a classic digestive hormone. Gastrin is mainly expressed in the G cells of the gastric mucosa. The release of gastrin is induced by nutrients and gastrin-releasing peptide, and is suppressed via somatostatin by high concentrations of H+ in the gastric mucosa. Clinically, gastrin is involved in Zollinger-Ellison syndrome, an ectopic gastrin-secreting tumor in the duodenum or pancreas. |
| Standard Curve | This product has been tested by Quality Control Department and meets performance specifications mentioned in the manual. (The humidity in the laboratory is 20%-60%, and the temperature is 18°C-25°C. TMB was balanced to 37°C before color development, and incubated at 37°C for 15 minutes in the dark after adding the enzyme label plate holes.) The following assay data are provided for reference, since experimental environment and operation are different. The establishment of standard curve depends on your own assay. |
Gastrin, as a key hormone regulating gastric acid secretion and gastric mucosal growth, is closely related to various diseases due to its abnormal levels. By using Creative Diagnostics' Human Gastrin ELISA Kit, researchers can delve into the mechanism of gastrin in the occurrence and development of diseases such as Zollinger Ellison syndrome, atrophic gastritis, and gastrinoma. This kit is also a powerful tool in the field of drug development, which can be used to screen and evaluate new drugs targeting the gastric acid secretion pathway. Its robust performance enables it to easily handle various complex research scenarios, including clinical cohort studies and cell model analysis, providing accurate biomarker quantification data for a wide range of fields from basic scientific research to translational medicine.
Exceptional Sensitivity
This kit is engineered for high sensitivity, capable of detecting even low physiological levels of Gastrin. The excellent precision ensures that your results are consistent and reproducible across multiple experiments
Ready-to-Use Convenience and Streamlined Workflow
Designed with the researcher in mind, the kit provides all necessary components pre-optimized to reduce preparation time and minimize operational errors
High Specificity for Human Gastrin
The antibodies used in this kit have been selected for high specificity to human Gastrin, showing minimal cross-reactivity with related molecule
The Human Gastrin ELISA Kit stands out as a reliable, efficient, and user-friendly tool for the accurate quantification of human gastrin. Its high sensitivity, specificity, and broad application range make it invaluable for advancing research on gastrointestinal diseases, supporting clinical auxiliary diagnosis, and accelerating pharmaceutical development. By addressing the limitations of traditional detection methods, it provides a safe, cost-effective, and high-throughput solution that meets the needs of both academic and industrial users. Whether used to study gastrin's role in mucosal biology or to monitor disease progression, this kit delivers consistent, trustworthy results that drive progress in digestive health research and care.
A1: No. The kit is specifically designed for human gastrin, as it uses antibodies targeting human gastrin epitopes. Cross-reactivity with gastrin from other species is extremely low (≤0.1%), so the kit cannot provide accurate results for non-human samples. For animal gastrin detection, please select species-specific ELISA kits.
A2: If the sample's absorbance value exceeds that of the highest standard (100 pg/mL), it indicates the gastrin concentration is above the detection range. In this case, you should dilute the sample with the kit's provided sample dilution buffer (or blank serum) at a suitable ratio and re-test. When calculating the final concentration, multiply the measured value by the dilution factor. It is recommended to perform a pre-test with a small sample volume to determine the optimal dilution ratio and avoid over-dilution (which may reduce accuracy).
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