Sample
serum, plasma, cell culture supernatants, tissue homogenate
Detection Method
Competitive ELISA
Intended Use
For quantitative detection of Human FAM214A (Protein FAM214A) in serum, plasma, tissue homogenates and other biological fluids.
Contents of Kit
1. 96-well strip plate (Dismountable), 1 plate
2. Lyophilized Standard, 2 vials
3. Sample/Standard dilution buffer, 20 mL
4. Biotin-detection antibody (Concentrated), 60 uL
5. Antibody dilution buffer, 10 mL
6. HRP-Streptavidin Conjugate(SABC), 120 uL
7. SABC dilution buffer, 10 mL
8. TMB substrate, 10 mL
9. Stop solution, 10 mL
10. Wash buffer (25X), 30 mL
11. Plate Sealer, 5 pieces
12. Product Manual, 1 copy
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Precision
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Detection Range
0.78-50 ng/mL
Citations
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Ramos-Martinez, EM; Herrera-Ramirez, AC; et al. Isolation of cDNA from Jacaratia mexicana encoding a mexicain-like cysteine protease gene. GENE 502:60-68(2012).
FREIJE, JMP; DIEZITZA, I; et al. MOLECULAR-CLONING AND EXPRESSION OF COLLAGENASE-3, A NOVEL HUMAN MATRIX METALLOPROTEINASE PRODUCED BY BREAST CARCINOMAS. JOURNAL OF BIOLOGICAL CHEMISTRY 269:16766-16773(1994).
COA
Certificate of Analysis
