Human Factor VIII Matched Antibody Pair (ABPR-L007)

Regulatory status: For research use only, not for use in diagnostic procedures.

Write a review

Size
Sufficient reagent for 4 x 96 well plates
Sample
Plasma
Species Reactivity
Human
Intended Use
This antibody pair set comes with matched antibody pair to detect and quantify protein level of Human Factor VII
Contents of Kit
1. Capture Antibody (yellow): 0.4 ml of polyclonal affinity purified anti-FVIII antibody for coating plates.
2. Detecting Antibody (neutral): Four neutral-capped tubes each containing 10 ml of pre-diluted peroxidase conjugated polyclonal anti-FVIII antibody for detection of captured FVIII.
3. Sample Diluent (green): 100 ml bottle containing a green-coloured diluent optimised for dilution of samples. Store reagents at 2-8°C
Note: Reagents are sufficient for at least 4×96 well plates using recommended protocols.
Storage
2-8°C
General Description
Factor VIII (formerly referred to as antihemophilic globulin and Factor VIII:C) is a large glycoprotein (320 kDa) that circulates in plasma at approximately 200 ng/ml. Synthesized in the liver, the majority of Factor VIII is cleaved during expression, resulting in a heterogeneous mixture of partially cleaved forms of F.VIII ranging in size from 200-280 kDa. The F.VIII is stabilized by association with von Willebrand Factor to form a F.VIII-vWF complex required for the normal survival of F.VIII in vivo (t1/2 of 8-12 hours). F.VIII is a pro-cofactor that is activated through limited proteolysis by thrombin. In this process F.VIIIa dissociates from vWF to combine with activated Factor IX, calcium and a phospholipid surface where it is an essential cofactor in the assembly of the Factor X activator complex. Once dissociated from vWF, F.VIIIa is susceptible to inactivation by activated Protein C and by non-enzymatic decay. Hemophilia A is a congenital bleeding disorder resulting from an X-chromosome-linked deficiency of F.VIII. The severity of the deficiency generally correlates with the severity of the disease. Some Hemophiliacs (~10%) produce a F.VIII protein that is partially or totally inactive. The production of neutralizing antibodies to F.VIII also occurs in 5-20% of Hemophiliacs.

Citations


Have you cited ABPR-L007 in a publication? Let us know and earn a reward for your research.

Related Products


Customer Reviews


Write a review, share your experiences with others and get rewarded !
Product Name Cat. No. Applications Host Species Datasheet Price Add to Basket
Product Name Cat. No. Applications Host Species Datasheet Price Add to Basket

Online Inquiry

Name:
Phone: *
E-mail Address: *
Technology Interest:
Type of Organization:
Service & Products Interested: *
Project Description:
Verification code
Click image to refresh the verification code.

Online Inquiry

  Interested in larger quantities ? request a quote!
  Protocol may be improved. Please feel free to contact us to obtain the latest version.!

Ordering Information

Payment methods we support:
Invoice / Purchase Order
Credit card

OUR PROMISE TO YOU Guaranteed product quality expert customer support

Inquiry Basket