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The Estradiol (E2) ELISA Kit from Creative Diagnostics is a highly sensitive and specific tool designed for the quantitative detection of 17β-estradiol in human serum, plasma, and other biological samples. Utilizing a validated competitive enzyme-linked immunosorbent assay (ELISA) format, this kit provides researchers with a reliable method to obtain precise data crucial for studying reproductive endocrinology, oncology, and developmental biology.
There are two types of estradiol, alpha and beta, which are one type of estrogen. The highest content and strongest activity. Secreted by granulosa cells in ovarian follicles. Its metabolites are estrone and estriol. Containing 18 carbon atoms. Its target organs are the uterus, vagina, fallopian tubes, and pituitary gland. Can be used as a therapeutic agent for estrogen absorbed through the skin. Supplementing the deficiency of 17- β estradiol secreted by female ovaries can also avoid side effects caused by oral administration, such as breast pain, weight gain, hypertension, gallstones, and liver dysfunction. Estrogen can promote the synthesis of DNA, RNA, and various proteins within the corresponding tissues in cells.
Figure 1. Estradiol.
1. Height increase: seen in female precocious puberty, male breast development, ovarian bleeding, and cirrhosis.
2. Reduced: seen in ovarian tumors, molar pregnancy, intrauterine stillbirth, pregnancy induced hypertension syndrome, hypothalamic tumors, pituitary dysfunction, ovarian insufficiency, oophorectomy, delayed puberty, primary and secondary amenorrhea, menopause, oral contraceptives, etc.
3. Pathological causes of increased estradiol levels: 1) Ovarian diseases: ovarian granulosa cell tumors, ovarian embryomas, ovarian fat like cell tumors, sex hormone producing tumors, etc., all of which indicate pregnancy (especially twin or multiple pregnancies), estrogen secreting tumors, ovulation inducing drugs (such as chlorpheniramine), male feminization, ovarian tumors, and no evidence of ovarian hyperfunction, resulting in increased estradiol secretion. 2) Heart disease: myocardial infarction, angina pectoris, coronary artery stenosis. 3) Other: Systemic lupus erythematosus, cirrhosis, male obesity. [4]
4. Pathological reasons for decreased estradiol: 1) Ovarian diseases: absent or underdeveloped ovaries, primary ovarian failure, ovarian cysts. 2) Pituitary amenorrhea or infertility. 3) Others: Hypothyroidism or hyperthyroidism, Cushing's syndrome, Addison's disease, malignant tumors, widespread infections, renal insufficiency, focal lesions of the brain and pituitary gland, etc., can all cause a decrease in plasma estradiol.
Sample Compatibility
Sensitivity Range
Main Uses
Estradiol (E2) is the most potent and predominant form of estrogen in the human body. This steroid hormone plays a vital role in regulating the menstrual cycle, supporting reproductive tissues, maintaining bone density, and influencing cardiovascular health. Accurate measurement of estradiol levels is essential for evaluating ovarian function, monitoring fertility treatments, assessing menopausal status, and investigating hormone-dependent cancers such as breast cancer. The Estradiol ELISA Kit provides researchers with a robust, sensitive tool for quantifying this critical reproductive hormone. Its excellent performance characteristics make it suitable for both basic research and clinical investigation applications.
Product Specifications
Intended Use
Key Features of the Product
Performance
| Parameter | Details |
| Product name | Estradiol ELISA Kit |
| Sample | estradiol serum, plasma |
| Intended Use | The Estradiol ELISA is an enzyme immunoassay for the quantitative in vitro diagnostic measurement of Estradiol in serum and plasma. |
| General Description | When stored at 2-8°C unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date. For more detailed information, please download the following document on our website. |
Compared to alternative E2 detection tools, it highlights three key reasons for directly addressing research pain points:
Meeting the precision requirements of tumor research: Hormone sensitive cancer research requires detecting small E2 fluctuations (such as a reduction of 10-15 pg/mL after anti estrogen therapy). Its low CV ensures reliable capture of these subtle changes, which cannot be achieved by low precision assay kits.
Save time and sample size: With 3 hours of analysis time and a sample size of 25 μ L, it solves two key researcher challenges: (1) generating data faster for time sensitive drug trials; (2) Preserving a large amount of valuable samples (such as pediatric serum, rare patient derived cell supernatants) that may be wasted by certain methods (e.g. 100 μ L for certain RIA kits).
Exceptional Sensitivity
Capable of detecting physiological levels of estradiol across the full range of clinical concentrations, from post-menopausal to peak ovulatory levels.
High Specificity
Minimal cross-reactivity with related estrogen metabolites ensures accurate measurement of 17β-estradiol.
Streamlined Workflow
Pre-coated plates and optimized reagents enable rapid, reproducible results with minimal hands-on time.
The Human Estradiol ELISA Kit is an indispensable tool for endocrinology and oncology research, addressing the critical need for sensitive, specific, and accessible E2 quantification. Its ultra-high sensitivity enables low-concentration sample testing (e.g., postmenopausal cancer patients), while its multi-sample compatibility and rapid assay time support diverse research designs—from small-scale preclinical studies to large-scale clinical trial screening. Compared to traditional methods (RIA, MS), it offers superior cost-effectiveness and ease of use, without compromising on performance. Backed by rigorous quality control and comprehensive technical support, this kit empowers researchers to generate reliable data that advances our understanding of hormone-related diseases and accelerates the development of life-saving therapies. For anyone studying E2's role in health and disease, this kit is the optimal choice.
A1: 1. Centrifuge urine at 3000 rpm for 5 minutes to remove precipitates. 2. Mix 100 μL of supernatant with 100 μL of the included urine pretreatment buffer (contains β-glucuronidase to hydrolyze E2 conjugates). 3. Incubate at 37°C for 1 hour. 4. Dilute the treated sample 1:2 with sample dilution buffer before adding to the microplate.
A2: No—this kit is optimized for human E2. Animal E2 has subtle structural differences that may reduce antibody binding. The manufacturer offers a separate "Rodent Estradiol ELISA Kit" for animal studies; contact support for details.
A3: Common causes: 1. The HRP-conjugated antibody was not brought to room temperature (it must equilibrate for 30 minutes before dilution). 2. The TMB substrate was stored incorrectly (exposure to light or temperatures >8°C degrades it). 3. The stop solution was added too early (wait until the highest standard has an absorbance of 1.5–2.0 at 450 nm).
A4: Yes. Serum/plasma can be frozen at -80°C for up to 6 months; urine can be frozen at -20°C for up to 3 months. Avoid more than 2 freeze-thaw cycles (this denatures proteins and may alter E2 levels). Thaw samples at room temperature and centrifuge before use.
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