Sample
serum, plasma, cell culture supernatants, tissue homogenate
Detection Method
Competitive ELISA
Intended Use
For quantitative detection of Human ENHO (Adropin) in serum, plasma, tissue homogenates and other biological fluids.
Contents of Kit
1. 96-well strip plate (Dismountable), 1 plate
2. Lyophilized Standard, 2 vials
3. Sample/Standard dilution buffer, 20 mL
4. Biotin-detection antibody (Concentrated), 60 uL
5. Antibody dilution buffer, 10 mL
6. HRP-Streptavidin Conjugate(SABC), 120 uL
7. SABC dilution buffer, 10 mL
8. TMB substrate, 10 mL
9. Stop solution, 10 mL
10. Wash buffer (25X), 30 mL
11. Plate Sealer, 5 pieces
12. Product Manual, 1 copy
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Precision
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Detection Range
78-5000 pg/mL
Citations
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Hamasaki, H; Yanai, H; et al. The association between daily physical activity and plasma B-type natriuretic peptide in patients with glucose intolerance: a cross-sectional study. BMJ OPEN 5:-(2015).
Troy-Fioramonti, S; Demizieux, L; et al. Acute Activation of Cannabinoid Receptors by Anandamide Reduces Gastrointestinal Motility and Improves Postprandial Glycemia in Mice. DIABETES 64:808-818(2015).