Human Echinococcus IgG ELISA Kit

Sample

Serum

Species Reactivity

Human

Detection Method

iELISA

Intended Use

The Echinococcus granulosus ELISA kit is intended for the qualitative detection of IgG antibodies against Echinococcus granulosus (and Echinococcus multilocularis) in human serum.

Contents of Kit

1. WELL Breakable ELISA strips sensitized with Echinococcus granulosus hydatid fluid antigen 96 wells
2. DILB Dilution buffer (10 x) concentrate, coloured purple 50 ml
3. WASH Washing solution (10 x) concentrate 50 ml
4. ENZB Enzyme buffer 50 ml
5. STOP Stopping solution (0.5M K₃PO₄) 25 ml
6. CONTROL - Negative control serum (20 x), green cap 200 μl
7. CONTROL -/+ Weak positive control serum (cut off, 20 x), yellow cap 200 μl
8. CONTROL + Positive control serum (20 x), red cap 200 μl
9. CONJ Protein A - alkaline phosphatase conjugate (50 x), purple cap 300 μl
10. SUBS Phosphatase substrate (para-nitrophenylphosphate) 20 tablets
11. Multipipette reservoir, 25 ml 1 piece
12. Frame for ELISA 8-well holder 1 piece

Storage

Store the kit at 2° to 8°C (transport at ambient temperature), avoid long term exposure of the components to direct light. The expiry date and the lot number of the kit are printed on the side of the box. After initial opening, all reagents are stable until the expiry date when stored at 2-8°C.

Performance Characteristics

A sensitivity of 96% was found with 24 sera of patients suffering from cystic echinococcosis. A sensitivity of 89% was found with 19 sera of patients suffering from alveolar echinococcosis. Positive and doubtful results should be retested with the Echinococcus multilocularis-specific Em2-Em18 to identify the infecting Echinococcus species. A specificity of 97% was found with 119 sera of blood donnors (Swiss).

Precision

Repeatability were assessed by testing 2 human serum samples in 24 wells on 1 assay.
Reproducibility were assessed by testing the 2 human serum samples on 10 differents assays.

General Description

Cystic echinocccosis, also known as hydatid disease, is caused by the larval stage of Echinococcus granulosus, a tapeworm found in dogs. Sheep, cattle, goats, and pigs as intermediate hosts harbor hydatid cysts mainly in liver and lungs. Humans can be infected by accidentally ingesting tapeworm eggs upon contaminated soil, food or water. The parasite larvae subsequently develop into hydatid cysts mainly in the liver and lungs as well. The main symptoms appear when hydatid cysts grow large enough to cause organ dysfunction plus discomfort, pain, nausea, and vomiting. Diagnosis is based on imaging techniques, such as CT scans, ultrasonography, and MRIs to detect the presence of well-delineated cyst(s) in a person with a history of exposure and a positive result by serological testing.

General Notes

Cystic echinocccosis, also known as hydatid disease, is caused by the larval stage of Echinococcus granulosus, a tapeworm found in dogs. Sheep, cattle, goats, and pigs as intermediate hosts harbor hydatid cysts mainly in liver and lungs. Humans can be infected by accidentally ingesting tapeworm eggs upon contaminated soil, food or water. The parasite larvae subsequently develop into hydatid cysts mainly in the liver and lungs as well. The main symptoms appear when hydatid cysts grow large enough to cause organ dysfunction plus discomfort, pain, nausea, and vomiting. Diagnosis is based on imaging techniques, such as CT scans, ultrasonography, and MRIs to detect the presence of well-delineated cyst(s) in a person with a history of exposure and a positive result by serological testing.
The Echinococcus granulosus ELISA kit is intended for the qualitative detection of IgG antibodies against Echinococcus granulosus (and Echinococcus multilocularis) in human serum.