Objective: The purpose of this study was to evaluate serum myoglobin as a cardiac muscle marker for the detection of early cardiovascular disorder and to assess total Creatine Kinase(CK) and creatinekinase-MB (CK-MB) levels among patients with Type 2 Diabetes mellitus (T2DM). Methodology: In this study, 300 study subjects were divided into two groups; 200 subjects diagnosed with T2DM (ascertained as cases) and 100 healthy individuals (ascertained as control group). Results: Significant increases in the serum levels of myoglobin, glucose, HbA1c, total creatine kinase and creatine kinase isoenzyme MB ( CK-MB) were observed in diabetic patients compared to levels in healthy individuals. Besides, a significant increase in myoglobin, total CK and CK-MB levels was observed among diabetic patients with ischemic heart disease or hypertension. Moreover a strong positive correlation was observed between the duration of diabetes and the serum levels of myoglobin, total CK and CK-MB (r = 0.80, P = 0.00; r = 0.68, P = 0.030; and r=0.78, P= 0.000, respectively). Additionally, the present study showed significant positive correlations between the body mass index (BMI) of diabetic patients and the serum levels of myoglobin, total CK and CK-MB (r=0.77, P=0.000; r=0.65, P=0.028; and r=0.76, P=0.000. respectively). However, this study showed insignificant weak positive correlations between HbA1C and the serum levels of myoglobin, total CK and CK-MB (r=0.28, P=0.068; r=0.27, P=0.075; and r=0.37, P=0.066, respectively). Conclusion: The higher levels of myoglobin, CK and CK-MB may be indicative of progressive cardiovascular disease among diabetic patients.