Human CK-MB (Creatine Kinase MB) ELISA Kit (DEIA-FN283)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
serum, plasma, cell culture supernatants, tissue homogenate
Species Reactivity
Human
Intended Use
For quantitative detection of Human CK-MB (Creatine Kinase MB) in serum, plasma, tissue homogenates and other biological fluids.
Contents of Kit
1. 96-well strip plate (Dismountable), 1 plate
2. Lyophilized Standard, 2 vials
3. Sample/Standard dilution buffer, 20 mL
4. Biotin-detection antibody (Concentrated), 120 uL
5. Antibody dilution buffer, 10 mL
6. HRP-Streptavidin Conjugate(SABC), 120 uL
7. SABC dilution buffer, 10 mL
8. TMB substrate, 10 mL
9. Stop solution, 10 mL
10. Wash buffer (25X), 30 mL
11. Plate Sealer, 5 pieces
12. Product Manual, 1 copy
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Precision
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Detection Range
0.156-10 ng/mL
Sensitivity
0.094 ng/mL
Standard Curve

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References


Role of serum microRNA-499 as a diagnostic marker in acute myocardial infarction

COR ET VASA

Authors: Vengatapathy, Kuzhandai Velu; Ramesh, Ramasamy; Rajappa, Medha; Kulkarni, Shweta; Hanifa, Mohammad

Acute myocardial infarction is one of the leading causes of morbidity and mortality in developing countries such as India and even worldwide. Its etiology is complicated because of several confounding factors involved in its pathogenesis. Recently, MicroRNA has been recognized to play an important role in predicting the diagnosis and prognosis of myocardial infarction. MiR-499 plays a pivotal role in the recovery of cardiac cell, following injury. Since several studies have profiled several miRs in cardiovascular diseases and of which miR-499 is mainly expressed in the myocardium. Our study is primarily designed to explore the diagnostic role of miR-499 in AMI. The study included 60 AMI patients aged 30-60 years and an equal number of age and gender matched controls. All cases were taken from Mahatma Gandhi Medical College and Research Institute a tertiary healthcare set up and analyzed for miR-499 by RT-PCR, Lipid Profile, CK (NAC), CK-MB and high sensitivity cardiac Troponin T were analyzed by IFCC approved methods. Unpaired Student's test was performed to compare the mean of the cases with the controls. Pearson correlation was used to study the association of miR with conventional markers. Receiver Operating Characteristic curve was plotted to find out the sensitivity and specificity of miR-499 in AMI. Our study showed significantly high levels of miR-499 in AMI patients in comparison to the healthy controls (p = 0.012). MiR-499 levels positively correlated with hs-cnTnT (r = 0.582, p < 0.01) and CK-MB (r = 0.443, p < 0.01). ROC for miR-499 showed an AUC 0.974 with 93.33% sensitivity and 86.67% specificity which was comparatively higher than the current markers CK-MB (86.67% and 71.67%) and hs-cTnT (90.00% and 81.56%). When these biomarkers analyzed for combined sensitivity increases but the specifi city decreases. The results showed that miR-499 has a significant correlation and also had higher sensitivity and specifi city when compared to other conventional markers and thus it could serve as a better early diagnostic marker of AMI.

Are higher serum levels of myoglobin and creatine kinase enzymes indicative for progressive cardiovascular disease among patients with type 2 diabetes?

MEDICAL SCIENCE

Authors: Elfaki, Elyasa Mustafa; Al Rashid, Fauwaz Fahad; Ahmed, Hussain Gadelkarim

Objective: The purpose of this study was to evaluate serum myoglobin as a cardiac muscle marker for the detection of early cardiovascular disorder and to assess total Creatine Kinase(CK) and creatinekinase-MB (CK-MB) levels among patients with Type 2 Diabetes mellitus (T2DM). Methodology: In this study, 300 study subjects were divided into two groups; 200 subjects diagnosed with T2DM (ascertained as cases) and 100 healthy individuals (ascertained as control group). Results: Significant increases in the serum levels of myoglobin, glucose, HbA1c, total creatine kinase and creatine kinase isoenzyme MB ( CK-MB) were observed in diabetic patients compared to levels in healthy individuals. Besides, a significant increase in myoglobin, total CK and CK-MB levels was observed among diabetic patients with ischemic heart disease or hypertension. Moreover a strong positive correlation was observed between the duration of diabetes and the serum levels of myoglobin, total CK and CK-MB (r = 0.80, P = 0.00; r = 0.68, P = 0.030; and r=0.78, P= 0.000, respectively). Additionally, the present study showed significant positive correlations between the body mass index (BMI) of diabetic patients and the serum levels of myoglobin, total CK and CK-MB (r=0.77, P=0.000; r=0.65, P=0.028; and r=0.76, P=0.000. respectively). However, this study showed insignificant weak positive correlations between HbA1C and the serum levels of myoglobin, total CK and CK-MB (r=0.28, P=0.068; r=0.27, P=0.075; and r=0.37, P=0.066, respectively). Conclusion: The higher levels of myoglobin, CK and CK-MB may be indicative of progressive cardiovascular disease among diabetic patients.

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