Magic™ Rabbit Anti-Human BRCA1 Monoclonal antibody, clone 351396B22

Antibody Isotype

IgG

Clone

351396B22

Species Reactivity

Human

Immunogen

BRCA1 fusion protein

Conjugate

Application Notes

We recommend the following antibodies for sandwich immunoassay (Capture - Detection):
DMAB-CPT1077 - DMAB-CPT1078

General Notes

This product (Catalog No. DMAB-CPT1077) is a rabbit-derived monoclonal antibody raised against human BRCA1. It has been affinity purified by Protein A. BRCA1, also named as RNF53, plays a central role in DNA repair by facilitating cellular response to DNA repair. It is required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. BRCA1 acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. It is involved in transcriptional regulation of P21 in response to DNA damage. BRCA1 is required for FANCD2 targeting to sites of DNA damage. It may function as a transcriptional regulator. BRCA1 inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation. The antibody is specific to BRCA1. BRCA1 appears to produce multiple splice variants. BRCA1 is a nuclear protein with a molecular mass of 220 kDa. The present study describes the isolation and expression of two cDNAs of BRCA1, including a splice variant designated BRCA1D672-4095. BRCA1D672-4095 is generated by exclusion of exon 11 by in-frame splicing and produces a 97 kDa protein. In contrast to BRCA1, BRCA1D672-4095 localizes to the cytoplasm.
DMAB-CPT1077 is suitable for use in CBA(Cap), ELISA(Cap) applications.
DMAB-CPT1077 is supplied as a liquid formulation in PBS only. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Alternative Names

PSCP; IRIS; EC:2.3.2.27; BROVCA1; BRCC1; breast cancer 1, early onset; BRCA1; RNF53

Entrez Gene ID

UniProt ID