Sample
Serum, plasma, cell culture supernatant
Intended Use
The human Artemin (hARTN) Quantification kit provides a rapid and easy method for the quantitative determination of hARTN in cell culture supernatant, serum and plasma. The kit includes ready-to-use reagents necessary to analyse up to 88 samples in 2 hours.
Storage
The kit should be stored at +2...+6°C. Unopened, the kit will remain stable until the expiry date printed on the kit label. The expiry date of each unopened component is printed on the label of the individual component. After opening, the components should be used within 8 weeks (microwell plate desiccation recommended).
As hARTN may be unstable in low concentrations (diluted standard stock solution, standards and diluted samples), it is recommended to prepare standards and samples directly before the test procedure. hARTN standards can be stored at -20°C up to one week if frozen immediately after preparation. Thaw and use only once.
Precision
Intra-assay precision:

Inter-assay precision:

Detection Range
The detection range of the assay is from 30 pg/mL to 1920 pg/mL.
Detection Limit
The detection limit is 2 pg/mL to 8 pg/mL, defined by the minimum hARTN concentration deviating by 2 standard deviations (2SD) from that of the standard A. The test was performed by using 16 replicate determinations of standard A (blank) and standard B.
Citations
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