This kit is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human APOD / Apolipoprotein D in samples of Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of APOD / Apolipoprotein D as low as 0.63 nanograms per millilter.
Storage
Store all kit components at 4°C. Store Coated 96-well Strip Plate, Standard, Biotinylated Detection Antibody and HRP Conjugate at -20°C if the kit will not be used within 1 month. The Substrate should never be frozen. Once individual reagents are opened it is recommended that the kit be used within 1 month. Unused Strip Plate wells should be stored at -20°C in a sealed bag containing desiccant in order to minimize exposure to moisture. Do not use the kit beyond its expiration date.
Precision
Intra-Assay CV (<4.76%) ; Inter-Assay CV (<6.12%)
Detection Range
0.63–40 ng/ml
Sensitivity
Typically less than 0.63 ng/ml
Standard Curve
The following standard curve is an example only and should not be used to calculate results for tested samples. A new standard curve must be generated for each set of samples tested.
Citations
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