Human Anti-Varicella Zoster Virus (gE) Antibody IgG ELISA Kit

Sample

Serum

Species Reactivity

Human

Detection Method

ELISA

Intended Use

The ELISA kit is developed for titer measurement of Anti-Varicella Zoster Virus Antibody IgG in human serum.

Storage

The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C. The shelf life is 30 days from the date of opening.

General Description

Glycoprotein E (gE) is one of the known glycoproteins (gB, gC, gE, gH, gI, gK, gL) of VZV that is most abundantly expressed on the surface of virus and infected cells, playing an important role in viral replication and cell-to-cell spread. The strongly immunogenic gE can provide strong IgG signal in body fluid, which makes it ideal to be developed as an antigen for analysis of Immunogenicity in the development of VZV vaccine. Therefore, It's helpful to develop the Human Anti-Varicella Zoster Virus Antibody IgG Titer ELISA Kit (gE) to detection the IgG (gE) titer level in human serum during the clinical stage of vaccine development.
This assay kit utilizes an indirect ELISA method to measure the titer of Anti-Varicella Zoster Virus Antibody IgG. The microplate is first immobilized with Glycoprotein E (VZV), followed by the addition of samples which are then incubated and the wells washed. Secondary antibody HRP-Anti-Human IgG is then added to the plate and incubated before washing the wells again. Substrate is then loaded into the wells and the color development is monitored in proportion to the amount of antibody present. The reaction can be stopped by adding a stop solution and the intensity of the absorbance can be measured at 450 nm and 630 nm, with the OD value reflecting the amount of antibody bound.

Standard Curve

For qualitative detection of antibodies:

For determination of antibody titer:
Quality control data between dif erent plates should not be mixed, and negative and positive controls should be set for each test.