Human Anti-S. Pneumococcal vaccine (Pneumovax/CPS23) IgG ELISA Kit

Sample

Serum, Plasma

Detection Method

iELISA

Intended Use

The Human Anti-Pneumococcal CPS23 IgG ELISA Kit quantifies IgG antibodies against capsular polysaccharides (CPS) of 23 serotypes (Pneumovax vaccine) in human serum/plasma of vaccinated or normal hosts. This immunoassay is suitable for:
1. Determining immune status relative to controls;
2. Assessing efficacy of vaccines, including dosage, adjuvantcy, route of immunization and timing;
3. Qualifying and standardizing vaccine batches & protocols.
For research use only (RUO), not for diagnosis, cure or prevention of the disease.

Contents of Kit

1; Wash Solution Concentrate (100×); 10mL; 2-8°C
2; Sample Diluent Concentrate (20×); 10mL; 2-8°C
3; Anti-Human IgG HRP Conjugate Concentrate (100×); 0.15 mL; 2-8°C
4; Pneumococcal 23 Type CPS Coated Strip Plate; 8-well strips (12); 2-8°C
5; Anti-Pneumococcal Calibrators (1 U/ml); 0.65mL; 2-8°C
6; Anti-Pneumococcal Calibrators (2.5 U/ml); 0.65mL; 2-8°C
7; Anti-Pneumococcal Calibrators (5 U/ml); 0.65mL; 2-8°C
8; Anti-Pneumococcal Calibrators (10 U/ml); 0.65mL; 2-8°C
9; Human Anti-Pneumococcal 23 IgG Positive Control; 0.65mL. Human anti-CPS IgG; diluted in buffer with protein, detergents and antimicrobial. Net OD >0.5
10; Low NSB Sample Diluent (LNSB) Reduces; 30 mL. Buffer with protein, detergents and antimicrobial. Use as is for sample dilution.
11; TMB Substrate; 12 mL. Chromogenic substrate for HRP containing TMB and peroxide.
12; Stop Solution; 12 mL. Dilute sulfuric acid.

Storage

The microtiter well plate and all other reagents, if unopened, are stable at 2-8°C until the expiration date printed on the box label.

General Description

Streptococcus pneumoniae, or pneumococcus, a Gram-positive bacteria, is one of the most common causes, along with N. meningitides, of bacterial meningitis in adults and young adults,Pneumococcal strains have capsular polysaccharides (CPS) that act as a virulence factor for the organism; more than 90 different serotypes are known. Serotype specific antibodies against the CPS provide protection against the corresponding strains. The pneumococcal vaccine most commonly used today consists of purified polysaccharides from 23 serotypes (nonconjugated: Pneumovax by Merck).Pneumococcal conjugate vaccines (PCV) contain polysaccharides conjugated to diphtheria toxin CRM197, with three PCV vaccines currently available:Prevnar-7 or PCV-7 (Wyeth) is a 7-valent vaccine; Synflorix (GSK) is a 10-valent vaccine (PCV10); Prevnar-13(Pfizer) is 13-valent (PCV-13: 1, 3, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19A, 19F, 23F); each are conjugated to carrier protein CRM197.

General Notes

Streptococcus pneumoniae, or pneumococcus, a Gram-positive bacteria, is one of the most common causes, along with N. meningitides, of bacterial meningitis in adults and young adults,Pneumococcal strains have capsular polysaccharides (CPS) that act as a virulence factor for the organism; more than 90 different serotypes are known. Serotype specific antibodies against the CPS provide protection against the corresponding strains. The pneumococcal vaccine most commonly used today consists of purified polysaccharides from 23 serotypes (nonconjugated: Pneumovax by Merck).Pneumococcal conjugate vaccines (PCV) contain polysaccharides conjugated to diphtheria toxin CRM197, with three PCV vaccines currently available:Prevnar-7 or PCV-7 (Wyeth) is a 7-valent vaccine; Synflorix (GSK) is a 10-valent vaccine (PCV10); Prevnar-13(Pfizer) is 13-valent (PCV-13: 1, 3, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19A, 19F, 23F); each are conjugated to carrier protein CRM197.