Human Anti-Francisella tularensis ELISA Kit

Sample

Serum

Species Reactivity

Human

Intended Use

The Anti-Francisella tularens is an in vitro immunoassay kit for detection of IgG, IgA and IgM antibodies to the lipopolysaccharide (LPS) of Francisella tularensis in human serum.

Storage

Upon receipt, all test components have to be kept at 2-8 °C, preferably in the original kit box. After opening all kit components are stable for at least 2 months, provided proper storage.

Precision

Intra-assay coefficient of variation:

Sensitivity

1

General Description

Francisella tularensis is the causative agent of tularaemia (deer fly fever) and is transferred to man directly from animals (especially rodents) or their ectoparasites. Infections via contaminated food or water and aerosols may also occur. After a 3 to 5 days lasting incubation time the onset of disease shows rather uncharacteristic common symptoms like headache, rheumatism, fatigue and fever. In dependence of the place of infection the clinical course manifests as ulcero-glandular type (primary affect, swelling of lymph nodes), as oculo-glandular type (conjunctivitis) or as inner type of tularaemia. The pulmonary course of infection is characterised by pneumonia and pleuritis, the abdominal course of infection by diarrhea, splenomegaly and intermittent fever. Direct detection of Francisella tularensis is possible after culture via microscopy and immunofluorescence. Detection of specific antibodies built in infected persons about two weeks after infection becomes more and more important for the diagnosis of tularaemia in the routine laboratory (indirect hemagglutination, complementfixation, enzyme immunoassay).